Ni-NTA Preloaded Columns&Plates

①Paub meej cov khoom parameter

Khoom qeb: Ni-NTA kem / phaj rau protein peptide antibody qauv npaj thiab protein purification

Khoom siv: PP + Agarose gel

Kem ntim: 1ml, 3ml, 5ml, 6ml, 12ml thiab 2ml 96-qhov desalination thiab purification daim hlau

Muaj nuj nqi: cov khoom theem extraction ntawm protein polypeptide antibody, pom, adsorption, sib cais, extraction, purification thiab concentration ntawm lub hom phiaj kuaj

Specifications: 0.5ml / 1ml, 1ml / 3ml, 1.5ml / 3ml, 1.5ml / 5ml, 2.5ml / 5ml, 2ml / 6ml, 3ml / 6ml, 4ml / 12ml, 6ml / 12ml, 0.4ml×96.8ml ,ua×96

Ntim: 25 pieces / 1ml, 20 pieces / 3ml, 30 pieces / 6ml, 20 pieces / 12ml, 5 pieces / box ntawm nruab nrab chromatography kem, 1 daim / pob ntawm 96-qhov desalination thiab purification phaj

Cov khoom ntim: aluminium foil hnab lossis opaque aluminium foil hnab (yeem)

Lub thawv ntim: nruab nrab lub thawv lossis BML xim lub thawv (yeem)

LOGO kev cai: yog

Hom khoom: OEM / ODM

②Khoom piav qhia

BM life science Ni-NTA kem yog purified los ntawm protein affinity chromatography nrog agarose li crosslinking nruab nrab.Lub hauv paus ntsiab lus purification yog purify lub hom phiaj protein nrog tag los ntawm kev siv cov cuab yeej tshwj xeeb khi ntawm npib tsib xee ions chelated ntawm NTA thiab tag.Cov qauv no yooj yim rau kev khiav lag luam , loj npaum li cas ntawm purification, yooj yim rau loj-scale purification ntawm yuav tsum tau cov proteins.

Nickel chloride hauv Ni kem tuaj yeem khi rau cov protein nrog nws (histone) daim ntawv lo.Tom qab khi, lub hom phiaj ntawm cov protein tuaj yeem tau txais los ntawm imidazole elution.Packing tsis ntawm Ni kem hauv puas mais kev tshawb fawb.

Substrate: 4% lossis 6% cross-linked agarose;

Qhov nruab nrab qhov loj me: 90um (45-165um); Kev daws teeb meem: 20% ethanol tov;

PH stability: 2-12;

Resistant denaturants: 6M guanidine hydrochloride thiab 8M urea;

Tiv thaiv siab: <0.3mpa, 0.1mpa raug pom zoo;

Adsorption:> 20mg (27kDa) protein / ml;

Cia tej yam kev mob: 2-8℃.

BM life science Ni preloaded kem muab cov khoom ntawm 5 specifications, nrog rau 1, 3, 5, 6 thiab 12ml.1ml thiab 5ml yog pre-loaded kab ntawm nruab nrab siab chromatography, uas yuav ua tau tag nrho cov zoo ntawm lub nruab nrab siab siab kua theem purification system rau sai thiab npaum desalination thiab purification ntawm lom macromolecules.

③Khoom nta:

★ntau yam specifications: 1/3/6/12ml rau syringe, 1/5ml rau nruab nrab chromatography kem;

★siab kam rau siab: nruab nrab siab chromatography pre-ntsia kem siab siab mus txog 0.6mpa (6 bar, 87 psi);

★yooj yim siv: ruer interface, tuaj yeem siv tau los ua cov qauv thauj khoom ntxiv, tseem tuaj yeem txuas nrog lub koob txhaj tshuaj thiab lub twj tso kua mis peristaltic, tseem tuaj yeem txuas ncaj qha KTA, Agilent, Shimadzu, Dej thiab lwm yam kua theem purification systems;

★dav siv: purified nucleic acid, tshuaj tiv thaiv kab mob, sau npe protein, protein desalination.

Order Cov ntaub ntawv

Cat No. xim piav qhia specification (ml) packing

PNI001-1 liab / ntsuab NI nruab nrab siab chromatography pre-ntsia kem 1 5 / thawv

PNI001-2 pob tshab raj / liab npog NI syringe hom preloaded kem 1 25 / thawv

PNI003-1 pob tshab raj / liab npog NI syringe hom pre-loaded kem 3 20 / thawv

PNI005-1 liab / ntsuab NI nruab nrab chromatography pre-installed kem 5 5 / lub thawv

PNI006-1 pob tshab raj / liab cap NI syringe hom pre-loaded kem 6 30 / thawv

PNI012-1 pob tshab raj / liab npog NI syringe hom pre-loaded kem 12 20 / thawv

BM Life Science, tag nrho cov kab ntawm SPE kem raj yog tsim los ntawm kev kho mob qib polypropylene txhaj tshuaj molding; Lub sieve phaj yog sintered nrog ultra-ntshiab ultra-siab molecular hnyav polyethylene.sorbents yuav thoob ntiaj teb, thiab los ntawm kev soj ntsuam xyuas, zoo yog trustworthy; 10,000 qib huv rhiav ntau lawm, txheej txheem ntau lawm, ERP tswj, cov khoom zoo traceability; ntau yam khoom specifications kom tau raws li cov neeg xav tau kev pab; tag nrho peb cov khoom raug kho kom cov neeg muas zaub tau txais kev pab zoo dua ib-nres.

Cov yam ntxwv ntawm BM Life Science SPE series khoom

Technical zoo:

★Qee qhov SPE sorbents kev tshawb fawb thiab kev tsim kho thiab kev tsim khoom siv tshuab nrog kev ywj pheej ntawm kev txawj ntse txoj cai, SPE loading (hmoov faib, thauj khoom, ntim khoom) yog tag nrho automated

★Kev cia siab rau qhov tshwj xeeb zoo ntawm cov concentrated pwm CNC txhaj tshuaj molding kev lag luam hauv cov hlaws dej delta, kev sib koom ua ke thiab kev siv tau zoo ntawm cov peev txheej tau nce ob npaug ntawm cov peev txheej ntawm SPE kem kav, halved tus nqi ntawm pwm tsim thiab txhaj tshuaj molding, thiab zoo heev txhim kho cov khoom zoo

★Lub tuam txhab muaj ib tug tshwj xeeb ultrafine rau nplua hmoov tis technology, hmoov tis tag nrho nyob rau hauv ib tug tsis siv neeg, batchable, scalable txoj kev los xyuas kom meej cov khoom batch ntau ruaj khov.

★Pua pua lab ntawm R & D thiab muaj peev xwm ntau lawm rau sieve daim hlau thiab lim core tau txo cov nqi tsim khoom ntawm SPE mus rau qib qis tshaj.

★SPE sieve phaj yog ywj siab kiag li, nws txoj kab uas hla, tuab, aperture loj tuaj yeem xaiv thiab sib sau ua ke ntawm qhov xav tau

★lub tuam txhab muab qhov tseem ceeb rau kev tsim kho thev naus laus zis thiab kev txhim kho txuas ntxiv, tshwj xeeb tshaj yog Tip SPE, sieves dawb vaj huam sib luag inlaying SPE, 96-384-qhov phaj SPE nrog kev ywj pheej cov cuab yeej cuab tam, uas ua rau qhov sib txawv hauv Suav teb thiab mus txog qib ntiaj teb, xav txog. qhov tshwj xeeb zoo ntawm B&M Life Science hauv SPE teb.

Khoom zoo:

★Yooj yim rau kev khiav lag luam, nyob rau hauv qhov kev txiav txim ntawm lub ntiajteb txawj nqus muaj peev xwm ua tau zoo tshaj plaws ntau yam thiab zoo reproducibility

★Nws tuaj yeem txuag tus nqi ntawm cov khoom siv thiab cov khoom siv tau zoo heev yam tsis muaj SPE thiab cov khoom siv nqus tsev

★huv si sorbent tsis muaj kev cuam tshuam los ntawm cov keeb kwm yav dhau

★siab rov ua dua tus nqi ntawm 10 ~ 100ppm yog nyob rau hauv qhov zoo tshaj plaws ntawm 95% ~ 105%

★Nrog loj adsorption muaj peev xwm, nws yog superior rau lwm SPE kem hom nyob rau hauv Tuam Tshoj

★ruaj khov khoom zoo, zoo reproducibility, load txheeb ze tus qauv sib txawv (RSD) <5%

★Tsis ntshai ntawm qhov qhuav qhuav.Ntev thiab ntub dej yog tib yam hauv qhov kev ua yuam kev, txheeb ze tus qauv sib txawv (RSD) <0.05%

★peb cov khoom muaj nyob rau tib theem zoo li Waters / Agilent / Supelco thiab lwm lub tuam txhab

★Tus nqi ua tau zoo ntawm peb cov khoom yog qhov zoo tshaj plaws hauv ntiaj teb

Daim ntawv thov:

★Siv dav hauv av; Roj; Cov kua dej hauv lub cev (plasma / zis, thiab lwm yam); Khoom noj khoom haus thiab lwm yam

Kev cog lus zoo:

★Xyuas kom meej tias txhua yam khoom tsim nyog, txais yuav cov qauv kev tswj xyuas nruj, ua raws li kev tshuaj xyuas batch

★Xyuas kom meej tias txhua yam khoom tsis muaj kev cuam tshuam dawb paug, tus nqi rov qab zoo dua li cov kev cai hauv lub tebchaws, kom ncav cuag qib siab ntawm cov khoom zoo sib xws.

Kev cog lus ua haujlwm:

★Muab kev pabcuam kev tshaj lij pub dawb

Kev faib tawm ntawm SPE kab

Polymer matrix series SPE

Nrog spherical polymer li adsorbent, sorbent particle loj yog ntau yam, SPE kem txaus tus nqi yog ntau ruaj khov, uas yog classical polymer kem nyob rau hauv lub chaw soj nstuam, tag nrho cov series ntawm cov khoom tau lug siv nyob rau hauv cov zaub mov kuaj.Cov khoom sib npaug rau Waters 'HLBS, MAX's thiab MCX's.

Silica gel matrix series SPE

Classic silica gel matrix SPE kem, amorphous / spherical adsorbent, nrog cov nqi siab thiab kev ua haujlwm zoo los ntawm cov neeg siv khoom ntseeg.Cov khoom siv dav siv hauv tshuaj ntsuam xyuas.

Ntsis extraction/purification/concentration ntawm SPE

Nws yog hmuv pipetting ntaus ntawv uas muaj nuj nqi yog thov rau extract / purification enrichment lom cov qauv los yog lub tswv yim ntawm SPE, lub hom phiaj khoom pipetting hmuv sab saum toj ntxiv ib co C4 / C18 / silicon hmoov / magnetic hlaws dai / ProtingA (G) agarose gel sorbent, siv rau primer / genomic DNA plasmid PCR khoom / peptide protein / tshuaj tiv thaiv rau cov khoom lag luam / lim / extraction / desalting / purification / enrichment.

96/384-qhov phaj series SPE

Lub 96/384-zoo phaj series SPE tsim tshwj xeeb rau high-throughput qauv pretreatment.Ua kom tiav cov qauv ua ntej txheej txheem nrog lub tuam txhab lub orifice lim los yog tsis siv neeg workstation.

Tshwj xeeb nrhiav tau series SPE

Azo dye tshwj xeeb nrhiav kom pom kab: xaiv ultra-ntshiab diatomite muab tub lim; Tshwj xeeb sieve-phaj txaus tus nqi tswj kev siv tshuab, los ntawm kev kuaj xyuas hauv tsev thiab txawv teb chaws, OEM muab.

Tsis tas li ntawd, muaj graphitized carbon dub, acid alkali nruab nrab alumina, txiv maj phaub plhaub tshuab txais carbon, zib ntab nrhiav kem, tawv decolorization kem, plasticizer nrhiav kem……SPE kem, ntau SPE khoom thov nug.

Cov kauj ruam (coj Tip SPE ua piv txwv):

Kev ua haujlwm yog qhov sib txawv me ntsis nyob ntawm qhov kev tuav tswj mechanism ntawm sorbent nrog adsorption ntawm cov khoom lag luam lossis impurities.

1. Lub sorbent adsorbs lub hom phiaj khoom, tshem tawm impurities thiab eludes lub hom phiaj khoom

Txoj Cai:

loading qauv tu, rejection ntxuav, elution

Hom kev ua haujlwm ntawm cov txheej txheem no feem ntau muaj plaub theem:

(1) ua kom - tshem tawm cov impurities hauv Tip SPE thiab tiam ntawm qee qhov hnyav ib puag ncig;

(2) cov qauv thauj khoom - yaj cov qauv hauv ib qho hnyav, siv cov pipette los nqus cov Tip SPE thiab khaws cov khoom ntawm lub Tip;

(3) leaching - qhov siab tshaj plaws tshem tawm ntawm impurities thiab lwm yam khoom tsis yog hom phiaj;

(4) elution - elution thiab sau ntawm lub hom phiaj cov khoom nrog ib tug me me ntim ntawm hnyav.

2. Lub sorbent adsorbs impurities thiab tshem tawm cov khoom lag luam

Txoj Cai:

Adsorb impurities ntawm tus qauv thiab tshem tawm cov khoom lag luam

Hom kev rho tawm cov theem no feem ntau muaj peb kauj ruam:

(1).Ua kom - tshem tawm cov impurities hauv Tip SPE thiab tsim kom muaj qee qhov hnyav ib puag ncig.

(2) cov qauv thauj khoom - nqus cov Ntsis SPE nrog pipette thiab maj mam tshuab nws tawm.Lub sijhawm no, feem ntau ntawm cov hom phiaj sib txuas yuav raug tshuab tawm nrog cov qauv hauv qab kev daws teeb meem, thiab cov impurities yuav khaws cia rau ntawm Tip.Yog li ntawd, cov kauj ruam yuav tsum tau pib sau.

(3) Elution — eluting lub Cheebtsam nrog ib tug me me ntim ntawm hnyav thiab sau, combining cov sau cov kua.

Ntau specifications los yog customizations, txais tostxhua tus neeg tshiab thiab qub los nug, sib tham txog kev koom tes, nrhiav kev txhim kho!