Ukuhlolwa kwe-Nucleic acid empeleni kuwukuthola ukuthi ingabe ikhona yini i-nucleic acid (RNA) ye-coronavirus entsha emzimbeni wesifundo sokuhlolwa.I-nucleic acid yegciwane ngalinye iqukethe ama-ribonucleotide, futhi inombolo nokuhleleka kwama-ribonucleotide aqukethwe kumagciwane ahlukene kuhlukile, okwenza igciwane ngalinye licace.
I-nucleic acid ye-coronavirus entsha nayo ihlukile, futhi ukutholwa kwe-nucleic acid ukutholwa okuqondile kwe-nucleic acid ye-coronavirus entsha.Ngaphambi kokuhlolwa kwe-nucleic acid, kuyadingeka ukuthi kuqoqwe amasampula esikhwehlela somuntu oxoxwayo, i-swab yomphimbo, i-bronchoalveolar lavage fluid, igazi, njll., futhi ngokuhlola la masampula, kungatholakala ukuthi umgudu wokuphefumula wesifundo ungenwe amagciwane.Ukutholwa kwe-coronavirus nucleic acid entsha kuvame ukusetshenziselwa ukutholwa kwesampula yomphimbo.Isampula liyahlukaniswa futhi lihlanzwe, futhi kungenzeka ukuthi i-coronavirus entsha ye-nucleic acid ikhishwa kuyo, futhi amalungiselelo okuhlolwa aselungile.

Ukutholwa kwe-coronavirus nucleic acid entsha ikakhulukazi kusebenzisa ubuchwepheshe be-fluorescence quantitative RT-PCR, okuyinhlanganisela yobuchwepheshe be-fluorescence quantitative PCR kanye nobuchwepheshe be-RT-PCR.Enqubweni yokuthola, ubuchwepheshe be-RT-PCR busetshenziselwa ukuguqula ukuloba i-nucleic acid (RNA) ye-coronavirus entsha ibe yi-deoxyribonucleic acid (DNA);bese kusetshenziswa ubuchwepheshe be-PCR be-fluorescence quantitative ukuphindaphinda i-DNA etholiwe ngobuningi.I-DNA ephindiwe itholakele futhi ibhalwe ngophenyo locansi.Uma kune-nucleic acid entsha ye-coronavirus, ithuluzi lingakwazi ukubona isignali ye-fluorescent, futhi, njengoba i-DNA iqhubeka nokuphindaphinda, isignali ye-fluorescent iyaqhubeka nokwanda, ngaleyo ndlela ibona ngokungaqondile ukuba khona kwe-coronavirus entsha.