Nucleic aside ikuramo ni igikoresho gikoresha reagent ikuramo aside nucleic kugirango ihite yuzuza icyitegererezo cya nucleic aside.Ikoreshwa cyane mubice bitandukanye nkikigo gishinzwe kurwanya indwara, gusuzuma indwara zamavuriro, umutekano wo guterwa amaraso, kumenyekanisha ubutabera, gupima mikorobe y’ibidukikije, gupima umutekano w’ibiribwa, ubworozi bw’amatungo n’ubushakashatsi bw’ibinyabuzima bwa molekile.

1. Igabanijwe ukurikije ingano yicyitegererezo

1)Ikibanza cyamazi cyikora

Ikibanza cyamazi cyikora nigikoresho gikomeye cyane, gihita cyuzuza gutanga amazi no kwifuza, ndetse gishobora no gutahura byimazeyo ibyakuweho, gukuramo, no gutahura muguhuza imirimo nka amplification no gutahura.Gukuramo aside nucleique nuburyo bumwe gusa bwo gukoresha imikorere yayo, kandi ntibikwiriye ko hakorwa laboratoire isanzwe ya acide nucleic.Mubisanzwe bikoreshwa mubigeragezo bikenerwa muburyo bumwe bwikigereranyo hamwe nubwinshi bwikigereranyo (byibuze 96, muri rusange magana) icyarimwe.Gushinga urubuga no gukora byikora byikora bisaba amafaranga menshi ugereranije.

2)Acide acide nucleic acide

Igikoresho gito cyikora cyikora kigera ku ntego yo guhita ikuramo aside nucleic binyuze mu miterere yimikorere, kandi irashobora gukoreshwa muri laboratoire iyo ari yo yose.

2. Itandukaniro ukurikije ihame ryo gukuramo

1)Ibikoresho ukoresheje spin inkingi uburyo

Uburyo bwa centrifugal inkingi acide nucleicikuramo cyane ikoresha ikomatanya rya centrifuge nigikoresho cyikora cyikora.Ibyinjira mubisanzwe ni 1-12 byintangarugero.Igihe cyo gukora kirasa nigihe cyo gukuramo intoki.Ntabwo itezimbere imikorere yakazi kandi ihenze.Ubwoko butandukanye Ibikoreshwa mubikoresho ntabwo ari rusange, kandi birakwiriye gusa muri laboratoire nini nini ifite amafaranga ahagije.

2) Ibikoresho ukoresheje uburyo bwa magneti

Gukoresha amasaro ya magnetique nkuwitwara, ukoresheje ihame ryamasaro ya magnetiki adsorbing acide nucleic munsi yumunyu mwinshi hamwe nagaciro ka pH, no kubitandukanya na acide nucleic munsi yumunyu muke nagaciro ka pH, inzira yose yo gukuramo aside nucleique no kuyisukura bigerwaho no kwimuka amasaro ya magneti cyangwa kwimura amazi.Bitewe numwihariko wihame ryayo, irashobora gushushanywa muburyo butandukanye, bushobora gukurwa mumiyoboro imwe cyangwa kuva 8-96, kandi imikorere yayo iroroshye kandi byihuse.Bifata iminota 30-45 gusa yo gukuramo ingero 96, zitezimbere cyane Imikorere yubushakashatsi nigiciro gito cyemerera gukoreshwa muri laboratoire zitandukanye.Kugeza ubu nigikoresho nyamukuru ku isoko.