Mwepụta oge siri ike (SPE) bụ usoro mmịpụta anụ ahụ nke gụnyere mmiri mmiri na akụkụ siri ike.N'ime usoro mmịpụta ahụ, ike mgbasa ozi nke siri ike na nyocha dị ukwuu karịa mmanya mmanya nne.Mgbe sample gafere site naSPEkọlụm, analyte na-adsorbed n'elu siri ike, na ihe ndị ọzọ na-agafe na kọlụm na sample nne mmanya.N'ikpeazụ, a na-ewepụ ihe nyocha ahụ na ihe mgbaze kwesịrị ekwesị Eluted.SPE nwere ngwa ngwa dị iche iche, dị ka nyocha nke mmiri mmiri dị ndụ gụnyere ọbara, mmamịrị, serum, plasma na cytoplasm;nyocha nke nhazi mmiri ara ehi, mmanya, ihe ọṅụṅụ na ihe ọṅụṅụ mkpụrụ osisi;nyocha na nlekota oru mmiri;mkpụrụ osisi, akwụkwọ nri, ọka, na ihe ọkụkụ dị iche iche anụ ahụ anụmanụ;ọgwụ siri ike dị ka mbadamba nkume.Nyocha nke pesticide na ahịhịa ahịhịa na mkpụrụ osisi, akwụkwọ nri na nri, nyocha nke ọgwụ nje na ọgwụ ụlọ ọgwụ, wdg.

(1) Jiri nlezianya wepụta ngwaọrụ mmịpụta oge siri ike ma tinye ya nwayọ na bench.

(2) Jiri nlezianya wepụ ihe mkpuchi elu nkeSPEngwaọrụ (jiri nwayọọ mee ka ọ ghara imebi obere tube), tinye tube ule ọkọlọtọ n'ime oghere nke nkebi na oghere oghere, wee kpuchie mkpuchi elu nke akọrọ, ma hụ na a na-eduzi mkpuchi ahụ ala.Igwe mmiri na-asọ asọ na tube ule na-edekọ otu otu, na mgbanaka akara nke square nke ihe mkpuchi ahụ nwere ezigbo akara ngosi na oghere oghere.Ọ bụrụ na ọ naghị adị mfe akara, enwere ike iji eriri roba mee ka ọ dịkwuo ike.

(3) Ọ bụrụ na ị zụtala ngbanwe nke onwe, ị ga-ebu ụzọ tinye valvụ ngbanwe n'ime oghere mmịpụta nke mkpuchi;

(4) Ọ bụrụ na ịchọghị ịme nlele 12 ma ọ bụ 24 n'otu oge, gbanye valvụ siri ike nke agịga n'ime oghere mmịpụta ejighi ya;

(5) Ọ bụrụ na azụrụ valvụ njikwa onwe ya, gbanye valvụ njikwa nke oghere mmịpụta ejighi ya na steeti akara kehoraizin;

(6) Fanye katrijị mmịpụta nke siri ike n'ime oghere mmịpụta ma ọ bụ oghere valve nke mkpuchi elu (tụgharịa eriri valve na-achịkwa na steeti mepere emepe kwụ ọtọ);jikọọ ngwaọrụ mmịpụta na mgbapụta agụụ na eriri, ma mee ka valvụ na-achịkwa nrụgide siri ike;

(7) Tinye ihe nlele ma ọ bụ reagents ka a na-amịpụta n'ime kọlụm mmịpụta, wee malite mgbapụta agụụ, mgbe ahụ ihe nlele na kọlụm mmịpụta ga-agafe na kọlụm mmịpụta na tube ule dị n'okpuru n'okpuru nrụgide na-adịghị mma.N'oge a, enwere ike ịhazigharị ọsọ ọsọ nke mmiri mmiri na-achịkwa site n'ịgbanwe nrụgide na-ebelata valvụ.

(8) Mgbe mmiri mmiri dị na tube agịga na-agbanyụ, gbanyụọ mgbapụta ahụ, wepụ ihe kọlụm na-emepụta ihe na ngwaọrụ ahụ, wepụ ihe mkpuchi elu nke ngwaọrụ ahụ, wepụ tube ule ma wụsa ya.

(9) Ọ bụrụ na ịchọghị iji tube ule jikọọ mmiri mmiri, ị nwere ike wepụ tube test, tinye ya n'ime akpa nke kwesịrị ekwesị, ma wepụ ya mgbe mbụ mmịpụta.

(10) Tinye tube ule dị ọcha n'ime ngwaọrụ ahụ, mechie mkpuchi, fanye katrij SPE, gbakwunye ihe mgbaze achọrọ na tube agịga, malite mgbapụta mgbapụta, gbanyụọ ike mgbe mmiri gwụchara, wee wepụ ya. ule tube maka ojiji.Mwepụta na nkwadebe sample zuru ezu.

(11) Tinye tube ule n'ime ngwa ihicha nitrogen ma mee ka ọ dị ọcha ma tinye uche na nitrogen, na nkwadebe zuru ezu.

(12) Tụfuo ihe mgbaze n'ime tube ule, ma sachaa tube ule maka ijikwa ya.

(13) Iji chekwaa ego eji eme iheSPEkọlụm, mgbe ejiri ya ọ bụla, a ga-ehichapụ kọlụm SPE na eluent iji hụ na njirimara nke nkwakọ ya.