Solid phase m'zigawo (SPE) ndi thupi m'zigawo ndondomeko monga madzi ndi olimba magawo.Mu ndondomeko m'zigawo, mphamvu adsorption olimba kwa analyte ndi wamkulu kuposa chitsanzo mayi mowa.Pamene chitsanzo chikudutsa muSPEndime, analyte ndi adsorbed pa olimba pamwamba, ndi zigawo zina kudutsa ndime ndi chitsanzo mayi mowa.Pomaliza, analyte amachotsedwa ndi chosungunulira choyenera Eluted.SPE ili ndi ntchito zambiri, monga kusanthula kwamadzimadzi achilengedwe kuphatikizapo magazi, mkodzo, seramu, plasma ndi cytoplasm;kusanthula kachulukidwe ka mkaka, vinyo, zakumwa ndi timadziti ta zipatso;kuwunika ndi kuyang'anira momwe madzi alili;zipatso, ndiwo zamasamba, mbewu, ndi mitundu yosiyanasiyana ya zomera Nyama zanyama;mankhwala olimba monga mapiritsi.Kusanthula kwa mankhwala ophera tizilombo ndi zotsalira za herbicide mu zipatso, ndiwo zamasamba ndi zakudya, kusanthula maantibayotiki ndi mankhwala azachipatala, etc.

(1) Mosamala chotsani chipangizo chochotsa gawo lolimba ndikuchiyika mofatsa pa benchi yogwirira ntchito.

(2) Chotsani chivundikiro chapamwamba chaSPEchipangizo (gwirani mofatsa kuti zisawononge chubu chaching'ono), ikani chubu choyezera choyezera mu dzenje la gawo la chipinda cha vacuum, ndiyeno phimbani chivundikiro chapamwamba chouma, ndikuonetsetsa kuti chivundikirocho chikuwongoleredwa pansi.Chubu chotuluka ndi chubu choyesera chimayenderana chimodzi ndi chimodzi, ndipo mphete yosindikizira ya mbale yophimba imakhala ndi ntchito yabwino yosindikizira ndi chipinda chowululiramo.Ngati sikophweka kusindikiza, ikhoza kumangirizidwa ndi gulu la rabara kuti muwonjezere kulimba.

(3) Ngati mwagula kusintha kodziyimira pawokha, muyenera choyamba kuyika valavu yosinthira mu dzenje lochotsa chivundikirocho;

(4) Ngati simukuyenera kuchita zitsanzo 12 kapena 24 panthawi imodzi, ikani valavu ya singano mu dzenje losagwiritsidwa ntchito;

(5) Ngati valavu yodziyimira payokha yagulidwa, tembenuzirani ndodo yowongolera ya dzenje losagwiritsidwa ntchito kumalo osindikizira opingasa;

(6) Ikani katiriji yolimba yotulutsa gawo mu dzenje lobowola kapena dzenje la valavu la chivundikiro chapamwamba (tembenuzani ndodo yowongolera kuti ikhale yotseguka);gwirizanitsani chipangizo chochotsa ndi pampu ya vacuum ndi payipi, ndikulimbitsa valavu yoyendetsera mphamvu;

(7) jekeseni zitsanzo kapena reagents kuti yotengedwa mu ndime m'zigawo, ndi kuyamba vacuum mpope, ndiye chitsanzo mu ndime m'zigawo zikuyenda mu ndime m'zigawo kwa mayeso chubu m'munsimu pansi pa zochita za mavuto zoipa.Panthawiyi, kuthamanga kwamadzimadzi kumatha kusinthidwa ndikuwongoleredwa ndikusintha valavu yochepetsera kuthamanga.

(8) Madzi a mu chubu cha singano akapopedwa, zimitsani mpope wa vacuum, chotsani mzati wowonjezera pa chipangizocho, chotsani chivundikiro chapamwamba cha chipangizocho, tulutsani chubu choyesera ndikutsanulira.

(9) Ngati simukufuna kugwiritsa ntchito chubu choyezera kuti mulumikize madziwo, mutha kutulutsa choyikapo choyesera, ndikuchiyika m'chidebe cha kukula koyenera, ndikuchichotsa mutachotsa koyamba.

(10) Ikani chubu choyezera choyera mu chipangizocho, tsekani chivundikirocho, ikani katiriji ya SPE, onjezerani chosungunulira chofunikira pa chubu cha singano, yambitsani pampu ya vacuum, zimitsani mphamvu pambuyo poti madzi atsitsidwa, ndikuchotsani. test chubu kuti mugwiritse ntchito.The m'zigawo ndi chitsanzo kukonzekera uli wathunthu.

(11) Ikani chubu choyesera mu chipangizo chowumitsira nayitrogeni ndikuyeretsa ndi kuyika ndi nayitrogeni, ndipo kukonzekera kwatha.

(12) Tayani zosungunulira mu chubu choyesera, ndi kutsuka chubu choyesera kuti mugwiritsenso ntchito.

(13) Kuti mupulumutse mtengo wogwiritsa ntchitoSPEMzere, pambuyo pa ntchito iliyonse, ndime ya SPE iyenera kutsukidwa mosavuta kuti iwonetsetse kuti katundu wake ndi wotani.