Fumonisin Affinity Chromatography
I-Fumonisins yityhefu yomngundo eveliswa ziscythe, ngoku kukho izinto eziphuma kwi-28 ezaziwayo, Esinye sezifundo eziqhelekileyo kunye nezinzulu yi-FB1.Kufunyaniswe ukuba umbona kunye neemveliso zawo, ezifana nokutya kwezilwanyana, zingcoliseka lula yi-FB1.I-FB1 yeyona inamandla kwiiFumonisins kwaye inefuthe eliyingozi kwizilwanyana ezininzi.Uphononongo lubonise ukuba ihashe elimhlophe le-FB1 linokubangela ukuthamba kwesifo sobuchopho, i-swine pulmonary edema syndrome, ukongeza, isenokubangela umhlaza wommizo kunye nomhlaza wesibindi, umhlaza wesisu kunye nezinye izifo, ukufuywa kwezilwanyana kunye nempilo yabantu. ikwabalulekile.
Uluhlu oluphambili lwe-B & M yokufumanisa i-toxin ikholomu ekhethekileyo yokufumanisa i-immuno-affinity kunye nekholomu ekhethekileyo ye-FB1 (FB1) .Le kholamu inokukhetha ngokukhetha i-vomatoxin B1 (FB1) kwisisombululo sesampuli, ngoko ke umphumo wokucoca wekholomu unokujoliswa ngoku. , kwaye isampuli inokuvavanywa ngokuthe ngqo yi-HPLC emva kokuba ikholamu ihlanjululwe.
| Isicelo: |
| Umbona;isondlo;ioli etyiwayo, njl. |
| Usetyenziso oluqhelekileyo: |
| Isetyenziselwa ukuhlanjululwa kweFumonisin |
| i-substrates entsonkothileyo kunye nomda ophantsi.Uhlalutyo lobungakanani |
| ye-TLC/HPLC/GC/LC-MS/EIA; |
| Inokulinganisa ngokomgangatho nangobungakanani |
| isixa esishiyekileyo seFumonisin kumbona, ukutya, ioli etyiwayo |
| kunye nezinye iisampuli |
Ulwazi lweodolo
| Ii-sorbents | Ifom | Inkcazo | IiPC/pk | Ikati.No |
| Ikhatriji yokubona iFumonisin | Ikhatriji | 1mL | 25 | FB-IAC0001 |
| Ikhatriji yokubona iFumonisin | 3mL | 20 | FB-IAC0003 | |
| Ikholamu engenanto ye-affinity chromatography | 1mL, amaqhekeza amabini e-hydrophilic Frits | 100 | ACC001 | |
| Ikholamu engenanto ye-affinity chromatography | 3mL, amaqhekeza amabini e-hydrophilic Frits | 50 | ACC003 |
