I-Nucleic Acid Extraction System (I-Nucleic Acid Extraction System) iyinsimbi esebenzisa ama-reagents e-nucleic acid afanayo ukuze iqedele ngokuzenzakalelayo isampula ye-nucleic acid.Isetshenziswa kakhulu ezikhungweni zokulawula izifo, ukuxilongwa kwezifo zasemtholampilo, ukuphepha kokumpontshelwa igazi, ukuhlonza i-forensic, ukuhlola amagciwane emvelo, ukuhlolwa kokuphepha kokudla, ukufuywa kwezilwane kanye nocwaningo lwebhayoloji yamangqamuzana neminye imikhakha.
1. Indlela yokumunca, eyaziwa nangokuthi indlela yepayipi, iwukukhipha i-nucleic acid ngokuvala ubuhlalu kazibuthe nokudlulisa uketshezi.Ngokuvamile, ukudluliswa kwenziwa ngokulawula ingalo yerobhothi ngohlelo lokusebenza.Inqubo yokukhipha imi kanje:

I-1) I-Lysis: Engeza isisombululo se-lysis kusampula, futhi uqaphele ukuxuba nokusabela okugcwele kwesisombululo sokusabela ngokunyakaza komshini nokushisa, amaseli akhishwa, futhi i-nucleic acid ikhishwa.

2) I-Adsorption: Engeza ubuhlalu obuzibuthe kusampula ye-lysate, xuba kahle, futhi usebenzise ubuhlalu obuzibuthe ukuze ube nokuhambisana okuqinile kwama-nucleic acid ngaphansi kukasawoti omningi ne-pH ephansi ukuze ukhangise ama-nucleic acid.Ngaphansi kwesenzo sensimu kazibuthe yangaphandle, ubuhlalu kazibuthe buhlukaniswa nesixazululo., sebenzisa ithiphu ukuze ukhiphe uketshezi futhi ululahle ethangini likadoti, futhi ulahle ithiphu.

3) Ukugeza: Khipha indawo kazibuthe yangaphandle, buyisela ithiphu elisha bese wengeza isigcinalwazi sokuwasha, xuba kahle ukuze ususe ukungcola, futhi ukhiphe uketshezi ngaphansi kwesenzo samandla kazibuthe wangaphandle.

4) I-Elution: Susa inkambu kazibuthe yangaphandle, buyisela ithiphu elisha, engeza isivali se-elution, uhlanganise kahle, bese uhlukanisa i-nucleic acid eboshiwe kubuhlalu kazibuthe ukuze uthole i-nucleic acid ehlanzekile.
2. Indlela yebha kaMagnetic

Indlela yenduku kazibuthe ibona ukuhlukaniswa kwama-nucleic acid ngokulungisa uketshezi nokudlulisa ubuhlalu kazibuthe.Umgomo kanye nenqubo kuyafana naleyo yendlela yokumunca, kodwa umehluko yindlela yokuhlukanisa phakathi kobuhlalu kazibuthe noketshezi.Indlela yebha kazibuthe iwukuhlukanisa ubuhlalu kazibuthe kusukela kumfucumfucu ngokusebenzisa i-adsorption yentonga kazibuthe ukuya kobuhlalu kazibuthe, bese ibufaka oketshezini olulandelayo ukuze kubonakale ukukhishwa kwe-nucleic acid.