I-Solid Phase Extraction (SPE) iyisampula yobuchwepheshe bokwelapha ethuthukiswe kusukela maphakathi nawo-1980.Ithuthukiswa inhlanganisela ye-liquid-solid extraction kanye ne-chromatography ewuketshezi.Ikakhulukazi esetshenziselwa ukuhlukaniswa, ukuhlanzwa kanye nokunothisa amasampula.Injongo eyinhloko ukunciphisa ukuphazamiseka kwesampula ye-matrix nokuthuthukisa ukuzwela kokutholwa.

Ngokusekelwe kumbono we-liquid-solid chromatography, ubuchwepheshe be-SPE busebenzisa i-adsorption ekhethiwe kanye ne-elution ekhethiwe ukuze inothise, ihlukanise futhi ihlanze amasampuli.Kuyinqubo yokukhipha umzimba okuhlanganisa izigaba eziwuketshezi neziqinile;kungase futhi kulinganiswe ngokuthathwa njengenqubo elula ye-chromatographic.
Umdwebo wohlelo lwedivayisi yokukhipha isigaba esiqinile
I-SPE umgomo wokuhlukanisa we-liquid chromatography usebenzisa i-adsorption ekhethiwe kanye ne-elution ekhethiwe.Indlela evame ukusetshenziswa kakhulu ukudlulisa isisombululo sesampula esiwuketshezi nge-adsorbent, ugcine into ezohlolwa, bese usebenzisa isincibilikisi samandla afanele ukukhipha ukungcola, bese ukhipha ngokushesha into ezohlolwa ngenani elincane. i-solvent, ukuze kuzuzwe inhloso yokuhlukana ngokushesha, ukuhlanzwa nokuhlushwa.Kungenzeka futhi ukukhangisa ngokukhetha ukungcola okuphazamisayo futhi uvumele into elinganisiwe iphume;noma ukukhangisa ukungcola kanye nento elinganisiwe ngesikhathi esifanayo, bese usebenzisa isincibilikisi esifanelekile ukuze ukhethe ngokukhetha into elinganisiwe.
I-extractant yendlela yokukhipha isigaba esiqinile iqinile, futhi isimiso sayo sokusebenza sisekelwe eqinisweni lokuthi izingxenye okufanele zilinganiswe kanye nezingxenye eziphazamisayo ezihlala ndawonye kusampula yamanzi zinamandla ahlukene ku-ejenti yokukhipha isigaba esiqinile, ukuze bahlukene omunye komunye.I-ejenti yokukhipha isigaba esiqinile iyisigcwalisi esikhethekile esiqukethe i-C18 noma i-C8, i-nitrile, i-amino namanye amaqembu.