Ni-NTA Yabanjirije Inkingi & Amasahani

①Ibicuruzwa birambuye

Icyiciro cyibicuruzwa: Ni-NTA inkingi / isahani ya protein peptide antibody icyitegererezo no gutunganya protein

Ibikoresho: PP + Agarose gel

Ingano yinkingi: 1ml, 3ml, 5ml, 6ml, 12ml na 2ml 96-umwobo wo gusiba no gusukura

Imikorere: gukuramo icyiciro gikomeye cya protein polypeptide antibody, kuyungurura, adsorption, gutandukana, gukuramo, kweza no kwibanda kubiteganijwe.

Ibisobanuro: 0.5ml / 1ml, 1ml / 3ml, 1.5ml / 3ml, 1.5ml / 5ml, 2.5ml / 5ml, 2ml / 6ml, 3ml / 6ml, 4ml / 12ml, 6ml / 12ml, 0.4ml×96, 0.8ml×96

Gupakira: ibice 25 / 1ml, ibice 20 / 3ml, ibice 30 / 6ml, ibice 20 / 12ml, ibice 5 / agasanduku k'inkingi yo hagati ya chromatografiya, igice 1 / ipaki ya 96-umwobo wo gusiba no gusukura

Ibikoresho byo gupakira: umufuka wa aluminiyumu cyangwa umufuka wa aluminiyumu wuzuye (utabishaka)

Agasanduku ko gupakira: agasanduku kitagira aho kibogamiye cyangwa agasanduku k'ibara rya BML (bidashoboka)

Umugenzo wa LOGO: yego

uburyo bwo gutanga: OEM / ODM

②Ibisobanuro ku bicuruzwa

Ubuzima bwa BM siyanse Ni-NTA inkingi yahanaguwe na protein affinity chromatografiya hamwe na agarose nkibikoresho bihuza.Ihame ryo kweza ni ugusukura poroteyine yagenewe hamwe na tagi ukoresheje umutungo wihariye uhuza hagati ya nikel ion yashizwe kuri NTA na tag.Ubu buryo bworoshye gukora , ubwinshi bwo kwezwa, byoroshye kurwego runini rwo kweza poroteyine zisabwa.

Nickel chloride muri Ni inkingi irashobora guhambirwa kuri proteine ​​hamwe na label ye (histone).Nyuma yo guhambira, poroteyine isanzwe irashobora kuboneka mugukuraho imidazole.Gupakira ibipimo bya Ni inkingi mubuzima bwa kilometero ijana siyanse yubuzima.

Substrate: 4% cyangwa 6% ihuza agarose;

Impuzandengo y'ibice: 90um (45-165um); Igisubizo cyo kubika: 20% igisubizo cya Ethanol;

PH itajegajega: 2-12;

Kwanga kwangirika: 6M guanidine hydrochloride na 8M urea;

Kurwanya igitutu: <0.3mpa, 0.1mpa birasabwa;

Adsorption:> 20mg (27kDa) proteine ​​/ ml;

Imiterere yo kubika: 2-8℃.

Ubuzima bwa BM Ubuzima Ni preloaded inkingi itanga ibicuruzwa byibisobanuro 5, harimo 1, 3, 5, 6 na 12ml.1ml na 5ml ni inkingi zabanje kwishyiriraho chromatografi yumuvuduko ukabije, zishobora gukoresha neza ibyiza bya sisitemu yo mu cyiciro cyo hagati yo kwisukura kugirango isukure vuba kandi neza no kweza macromolecules.

③Ibiranga ibicuruzwa:

★ibisobanuro bitandukanye: 1/3/6 / 12ml ya syringe, 1/5ml kuri chromatografi yo hagati;

★umuvuduko mwinshi wo kwihanganira: igitutu giciriritse chromatografi yabanje gushyirwaho igitutu cyo kwihanganira inkingi igera kuri 0,6mpa (6 bar, 87 psi);

★byoroshye gukoresha: Imigaragarire ya ruer, irashobora gukoreshwa murukurikirane kugirango yongere urugero rwikigereranyo, irashobora kandi guhuzwa na syringe na pompe ya peristaltike, irashobora kandi guhuzwa na KTA, Agilent, Shimadzu, Amazi nubundi buryo bwo kweza ibyiciro byamazi;

★ikoreshwa cyane: aside nucleic isukuye, antibodies, yanditseho protein, protein desalination.

Order Amakuru

Injangwe No ibara risobanura ibisobanuro (ml) gupakira

PNI001-1 umutuku / icyatsi NI urwego rwumuvuduko wa chromatografiya yashizwemo mbere inkingi 1 5 / agasanduku

PNI001-2 umuyoboro utagaragara / igipfukisho gitukura NI syringe ubwoko bwabanjirije inkingi 1 25 / agasanduku

PNI003-1 umuyoboro utagaragara / igipfukisho gitukura NI syringe ubwoko bwabanje kwishyiriraho inkingi 3 20 / agasanduku

PNI005-1 umutuku / icyatsi NI iciriritse chromatografiya yashyizweho mbere inkingi 5 5 / agasanduku

PNI006-1 umucyo utagaragara / umutuku cap NI syringe ubwoko bwabanje gupakirwa inkingi 6 30 / agasanduku

PNI012-1 umuyoboro utagaragara / igipfukisho gitukura NI syringe ubwoko bwabanje gupakirwa inkingi 12 20 / agasanduku

Ubuzima bwa BM Ubuzima, urukurikirane rwose rwibikoresho bya SPE bikozwe muburyo bwo kwa muganga bwa polypropilene yo guterwa inshinge; isahani ya sikeri yandujwe na ultra-pure ultra-high-molecular polyethylene.sorbents yaguzwe kwisi yose, kandi binyuze mubisuzuma byemewe, ubuziranenge bwizewe 10,000; icyiciro cyo gukora amahugurwa asukuye, uburyo busanzwe bwo gukora, imicungire ya ERP, ubuziranenge bwibicuruzwa bikurikirana; ibicuruzwa bitandukanye bitandukanye kugirango uhuze ibyo umukiriya akeneye; ibicuruzwa byacu byose byateganijwe kugirango abakiriya bishimira serivisi nziza yo guhagarara rimwe.

Ibiranga ubuzima bwa BM Ubumenyi bwa SPE ibicuruzwa

Ibyiza bya tekiniki:

★Bamwe muri SPE sorbents ubushakashatsi niterambere hamwe nubuhanga bwo gukora hamwe nuburenganzira bwumutungo wubwenge wigenga, gupakira SPE (gukwirakwiza ifu, gupakira, gupakira) byikora byuzuye

★Hashingiwe ku nyungu zidasanzwe z’inganda zashizwemo n’inganda za CNC mu masoko y’uruzi rwa pearl, guhuza no gukoresha neza umutungo byikubye kabiri umusaruro w’imiyoboro ya SPE inkingi, bikagabanya kabiri ikiguzi cyo gukora ibumba no kubumba inshinge, kandi byateje imbere cyane ubuziranenge bwibicuruzwa

★Isosiyete ifite ultrafine idasanzwe yo gukwirakwiza ifu nziza, gukwirakwiza ifu rwose muburyo bwikora, bworoshye, bwagutse kugirango ibicuruzwa bigerweho neza

★Amamiriyoni ya R&D hamwe nubushobozi bwo kubyaza amasahani hamwe nayunguruzo byagabanije igiciro cyumusaruro wa SPE kurwego rwo hasi

★Isahani ya SPE yamashanyarazi irigenga rwose, diameter, ubunini, ubunini bwa aperture irashobora gutoranywa no gukusanyirizwa uko bishakiye

★isosiyete iha agaciro kanini udushya mu ikoranabuhanga no gukomeza gutera imbere, cyane cyane Impanuro ya SPE, ikuraho akanama k'ubuntu karimo SPE, 96-384-isahani ya plaque SPE ifite uburenganzira ku mutungo bwite w’ubwenge, wuzuza icyuho mu Bushinwa kandi ukagera ku rwego rw’isi, bikagaragaza ibyiza bidasanzwe bya B&M Ubumenyi bwubuzima murwego rwa SPE.

Ibyiza byibicuruzwa:

★Biroroshye gukora, munsi yibikorwa bya rukuruzi karemano birashobora kugera kumuvuduko mwinshi kandi byororoka neza

★Irashobora kuzigama ikiguzi cyibikoresho nibikoreshwa cyane nta SPE nibikoresho bya vacuum

★isuku sorbent nta kubangamira inyuma yubusa

★igipimo kinini cyo gusubiramo cya 10 ~ 100ppm cyari murwego rwiza rwa 95% ~ 105%

★Nubushobozi bunini bwa adsorption, iruta izindi marike ya SPE mubushinwa

★ibicuruzwa byiza bihamye, kubyara neza, umutwaro ugereranije gutandukana bisanzwe (RSD) <5%

★Ntutinye inkingi yumye.Ibishishwa byumye kandi bitose ni bimwe murwego rwamakosa, ugereranije gutandukana bisanzwe (RSD) <0.05%

★ibicuruzwa byacu biri murwego rwose rwiza nkamazi / Agilent / Supelco nandi masosiyete

★Igipimo cyibikorwa byibicuruzwa byacu biri mubyiza kwisi

Gusaba:

★Ikoreshwa cyane mubutaka; Amavuta; Amazi yumubiri (plasma / inkari, nibindi.); Ibiryo nibindi

Ubwitange bufite ireme:

★Menya neza ko ibicuruzwa byose byujuje ibisabwa, kwemeza ibipimo ngenderwaho bigenzura ubuziranenge, gushyira mu bikorwa igenzura

★Menya neza ko buri gicuruzwa kitagira icyo kibangamira, igipimo cyo gukira kiruta amategeko y’igihugu, kugira ngo ugere ku rwego rwo hejuru rw’ibicuruzwa bisa

Imihigo yo gukora:

★Tanga inkunga ya tekiniki yumwuga kubuntu

Itondekanya rya SPE inkingi

Polymer matrix ikurikirana SPE

Hamwe na polymer spherical nka adsorbent, ingano ya sorbent ingano irasa cyane, umuvuduko winkingi ya SPE urahagaze neza, akaba aribwo buryo bwa polymer busanzwe muri laboratoire, ibicuruzwa byose byakoreshejwe cyane mugupima ibiryo.Ibicuruzwa bihwanye n’amazi HLBS, MAX na MCX.

Silica gel matrix ikurikirana SPE

Classic silica gel matrix SPE inkingi, amorphous / spherical adsorbent, hamwe nibikorwa bihendutse hamwe nuburambe bwiza bwabakoresha kubwizere bwabakiriya.Ibicuruzwa bikoreshwa cyane mubisesengura ryimiti.

Gukuramo inama / kweza / kwibanda kuri SPE

ni igikoresho cyo gucamo icumu gifite imikorere ikoreshwa mugukuramo / kweza bikungahaye ku binyabuzima cyangwa Impanuro ya SPE, ibicuruzwa biva mu icumu hejuru yongeweho bimwe bya C4 / C18 / ifu ya silicon / amasaro ya magneti / ProtingA (G) agarose gel sorbent, ikoreshwa kuri primer / genomic ADN plasmid PCR / peptide protein / antibodies kugirango igere ku bicuruzwa / muyungurura / gukuramo / gusiba / kweza / gutungisha.

96/384-umwobo wa plaque ya SPE

96/384-isahani yuruhererekane rwamasahani SPE yateje imbere muburyo bwihariye bwo kwinjiza ibicuruzwa byinshi. Uzuza icyitegererezo cyambere cyo gutunganya hamwe na filteri ya orifice ya sosiyete cyangwa aho ikorera.

Urutonde rwihariye rwo gutahura SPE

Azo irangi ryihariye rimenyekanisha inkingi: hitamo ultra-pure diatomite yuzuza; Ikoranabuhanga ridasanzwe ryo kugenzura ibipimo byerekana umuvuduko, binyuze mubigo byemewe byimbere mu gihugu ndetse n’amahanga, OEM itanga.

Mubyongeyeho, hariho ibishushanyo mbonera bya karubone, aside alkali itabogamye alumina, shell coconut shell ikora karubone, inkingi yo gutahura ubuki, inkingi ya decolorisation yimpu, inkingi yerekana plastike ink inkingi ya SPE, ibicuruzwa byinshi bya SPE nyamuneka ubaze.

Intambwe (fata Inama SPE nk'urugero):

Igikorwa kiratandukanye gato bitewe nuburyo bwo kugumana sorbent hamwe na adsorption yibicuruzwa bigenewe cyangwa umwanda

1. Sorbent yamamaza ibicuruzwa bigenewe, ikuraho umwanda kandi ikuraho ibicuruzwa bigenewe

Ihame:

gupakira icyitegererezo cyo gukora isuku, kwanga gukaraba, gukuraho

Ubu bwoko bwibikorwa byo gukuramo icyiciro muri rusange bifite intambwe enye:

(1) gukora - kuvanaho umwanda muri Tip SPE no kubyara ibidukikije bimwe na bimwe;

.

(3) gutobora - gukuraho cyane umwanda nibindi bicuruzwa bitagenewe;

(4) gukuraho - gukuraho no gukusanya ibicuruzwa bigenewe hamwe nubunini buke bwa solve.

2. Sorbent yamamaza umwanda kandi ikuraho ibicuruzwa bigenewe

Ihame:

Adsorb yanduye kurugero no kwirukana ibicuruzwa bigenewe

Ubu bwoko bwo gukuramo icyiciro gikomeye mubusanzwe bufite intambwe eshatu:

(1).Gukora - kuvanaho umwanda muri Tip SPE hanyuma ubyare ibidukikije runaka.

(2) gupakira icyitegererezo - guhumeka Impanuro SPE hamwe na pipette hanyuma uyihute buhoro.Muri iki gihe, ibyinshi mubyateganijwe bizashyirwa ahagaragara hamwe nicyitegererezo cyibanze, kandi umwanda uzagumana kuri Tip.Kubwibyo, intambwe zigomba gutangira gukusanya.

.

Ibisobanuro byinshi cyangwa byihariye kugiti cyawe, urakaza nezaabakiriya bose bashya nabakera kubaza, kuganira kubufatanye, gushaka iterambere rusange!