Ni-NTA Preloaded Columns&Plates

①Detailed chigadzirwa parameters

Chikamu chechigadzirwa: Ni-NTA koramu/ndiro yeprotein peptide antibody sampuli yekugadzirira uye kucheneswa kweprotein

zvinhu:PP+Agarose gel

Column volume: 1ml, 3ml, 5ml, 6ml, 12ml uye 2ml 96-gomba desalination uye mahwendefa ekuchenesa

Basa: yakasimba chikamu kudhirowa kweprotein polypeptide antibody, kusefa, adsorption, kupatsanurwa, kudhirowa, kucheneswa uye kuunganidzwa kwezvinangwa samples.

Zvinodiwa: 0.5ml/1ml, 1ml/3ml, 1.5ml/3ml, 1.5ml/5ml, 2.5ml/5ml, 2ml/6ml, 3ml/6ml, 4ml/12ml, 6ml/12ml,×96, 0.8ml×96

Packaging: 25 zvidimbu / 1ml, 20 zvidimbu / 3ml, 30 zvidimbu / 6ml, 20 zvidimbu / 12ml, 5 zvidimbu / bhokisi repakati chromatography column, 1 chidimbu / pakiti ye96-gomba desalination uye ndiro yekuchenesa

Packaging material: aluminium foil bag kana opaque aluminium foil bag (optional)

Packaging box: neutral label box kana BML color box (optional)

LOGO tsika: hongu

kugovera maitiro: OEM/ODM

②Chigadzirwa tsananguro

BM life science Ni-NTA column yakacheneswa neprotein affinity chromatography with agarose as crosslinking medium.Nheyo yekuchenesa ndeyekuchenesa mapuroteni ane tag nekushandisa chaiyo inosunga pfuma pakati penickel ions chelated paNTA uye tag.Iyi nzira iri nyore kushandisa. , kucheneswa kukuru, nyore kucheneswa kukuru kwemapuroteni anodiwa.

Nickel chloride muNi column inogona kusungirirwa kuprotein ine (histone) label.Mushure mekusunga, mapuroteni echisikigo anogona kuwanikwa nemidazole elution.Packing parameters yeNi column mumazana emamaira ehupenyu sainzi.

Substrate: 4% kana 6% cross-yakabatanidzwa agarose;

Avhareji yehukuru hwepakati: 90um (45-165um); Kuchengetedza mhinduro: 20% ethanol mhinduro;

PH kugadzikana: 2-12;

Resistant denaturants: 6M guanidine hydrochloride uye 8M urea;

Kutsungirira kumanikidzwa: <0.3mpa, 0.1mpa inokurudzirwa;

Adsorption:> 20mg (27kDa) protein / ml;

Kuchengetedza mamiriro: 2-8℃.

BM hupenyu sainzi Ni preloaded column inopa zvigadzirwa zve 5 zvakatemwa, zvinosanganisira 1, 3, 5, 6 uye 12ml.1ml uye 5ml ndiwo akafanorongedzerwa makoramu epakati pressure chromatography, anogona kushandisa zvizere zvakanakira yepakati pressure fluid chikamu chekuchenesa system yekukurumidza uye inoshanda desalination uye kucheneswa kwebiological macromolecules.

③Product features:

★zvinyorwa zvakasiyana-siyana: 1/3/6/12ml yesirinji, 1/5ml yepakati chromatography column;

★kushivirira kwakanyanya: yepakati kudzvanywa chromatography pre-yakaiswa mbiru kushivirira kumanikidza kusvika ku0.6mpa (6 bar, 87 psi);

★nyore kushandisa: ruer interface, inogona kushandiswa mumutsara kuwedzera sampuli kurodha, inogonawo kubatanidzwa kune sirinji uye peristaltic pombi, inogonawo kubatanidzwa zvakananga KTA, Agilent, Shimadzu, Mvura uye mamwe maitiro ekuchenesa chikamu chemvura;

★inoshandiswa zvakanyanya: yakacheneswa nucleic acid, masoja ekudzivirira chirwere, mapuroteni akanyorwa, mapuroteni desalination.

Order Information

Katsi Nhamba tsanangudzo yemavara (ml) kurongedza

PNI001-1 tsvuku / girini NI yepakati kumanikidza chromatography pre-yakaiswa mbiru 1 5 / bhokisi

PNI001-2 yakajeka chubhu / tsvuku chivharo NI sirinji mhando preloaded mbiru 1 25 / bhokisi

PNI003-1 yakajeka chubhu / tsvuku chivharo NI sirinji mhando pre-yakatakura mbiru 3 20 / bhokisi

PNI005-1 tsvuku / girini NI yepakati chromatography pre-yakaiswa column 5 5 / bhokisi

PNI006-1 yakajeka chubhu / tsvuku chivharo NI sirinji mhando pre-yakarodha mbiru 6 30 / bhokisi

PNI012-1 yakajeka chubhu / tsvuku chivharo NI sirinji mhando pre-yakatakura mbiru 12 20 / bhokisi

BM Hupenyu Sayenzi, ese akateedzana eSPE column machubhu akagadzirwa ekurapa-giredhi polypropylene jekiseni rekugadzira; Iyo sieve ndiro inosiiwa ne Ultra-pure-yakakwirira mamorekuru uremu polyethylene.sorbents inotengwa pasi rose, uye kuburikidza nemvumo yekuongorora, mhando yakavimbika; 10,000 giredhi yakachena workshop kugadzirwa, yakamisikidzwa kugadzirwa maitiro, ERP manejimendi, chigadzirwa chemhando yekuteedzera; zvakasiyana-siyana zvechigadzirwa zvinosangana nezvinodiwa nevatengi; zvigadzirwa zvedu zvese zvakagadzirirwa kuti vatengi vanakirwe nemhando yepamusoro-yekumira sevhisi.

Zvimiro zveBM Hupenyu Sayenzi SPE akatevedzana zvigadzirwa

Technical zvakanakira:

★Mamwe maSPE sorbents ekutsvagisa nekusimudzira uye kugadzira tekinoroji ine yakazvimirira kodzero yezvivakwa zvepfungwa, SPE kurodha (kugovera hupfu, kurodha, kurongedza) izere otomatiki.

★Tichivimba neakasarudzika mabhenefiti eiyo yakanamirwa mold CNC jekiseni rekuumba indasitiri muparera rwizi delta, kubatanidzwa uye nemashandisirwo ekushandisa zviwanikwa kwakawedzera zvakapetwa kugona kwekugadzira kweSPE column pombi, nepakati mutengo wekugadzira mold nekuumba jekiseni, uye yakavandudza zvakanyanya chigadzirwa chemhando

★Iyo kambani ine yakasarudzika ultrafine kune yakanaka yekugovera hupfu tekinoroji, kugovera hupfu zvachose mune otomatiki, batchable, scalable nzira yekuona kuti chigadzirwa batch yakagadzikana.

★Mazana emamiriyoni eR&D uye kugona kugadzira kwemapureti esieve uye sefa musimboti zvakadzikisa mutengo wekugadzira weSPE kusvika padanho rakaderera.

★SPE sieve ndiro yakazvimirira zvachose, dhayamita yayo, ukobvu, aperture saizi inogona kusarudzwa uye kugovaniswa pakuda.

★iyo kambani inokoshesa zvakanyanya kuhunyanzvi hwekuvandudza tekinoroji uye kuenderera mberi kwekuvandudza, kunyanya Tip SPE, sieves emahara mapanera inlaying SPE, 96-384-gomba ndiro SPE ine yakazvimirira kodzero yezvivakwa zvehungwaru, inozadza mukaha muChina uye inosvika padanho repasi rose, ichiratidza. iwo akasiyana mabhenefiti eB&M Hupenyu Sainzi mumunda weSPE.

Product zvakanakira:

★Chiri nyore kushanda, pasi pechiito chesimba rinokwevera pasi rinokwanisa kuwana yakanakisa velocity renji uye yakanaka kuberekana

★Inogona kuchengetedza mutengo wemidziyo uye zvinodyiwa zvakanyanya pasina SPE uye vacuum michina

★yakachena sorbent isina kukanganiswa kubva kuseri isina chinhu

★high recycle rate ye10 ~ 100ppm yaive mumhando yepamusoro ye95% ~105%

★Iine yakakura adsorption kugona, yakakwirira kune mamwe SPE column brands muChina

★kugadzikana kwemhando yechigadzirwa, kuberekana kwakanaka, kutakura chiyero chekutsauka (RSD) <5%

★Kusatya mbiru yakaoma. Kuyerera kwakaoma uye kunyorova kwakafanana mukati memhosho renji, chiyero chiyero chekutsauka (RSD) <0.05%

★zvigadzirwa zvedu zvakakwana pachiyero chemhando seMvura / Agilent/ Supelco nemamwe makambani

★Mutengo wekuita mwero wezvigadzirwa zvedu uri pakati pezvakanakisa pasirese

Application:

★Inoshandiswa zvakanyanya muvhu;Mafuta;zvinwiwa zvemuviri (plasma/weti, etc.);Chikafu zvichingodaro.

Kuzvipira kwehutano:

★Ita shuwa kuti chigadzirwa chose chinokodzera, tora yakasimba yemhando yekudzora zviyero, shandisa batch kuongorora

★Ita shuwa kuti chigadzirwa chega chega hachina kupindira kusina chinhu, chiyero chekudzorera chiri nani pane mirairo yenyika, kusvika padanho repamusoro rezvigadzirwa zvakafanana.

Zvisungo zvekuita:

★Ipa rubatsiro rwehunyanzvi hwehunyanzvi mahara

Kurongwa kwe SPE column

Polymer matrix akatevedzana SPE

Iine spherical polymer se adsorbent, sorbent particle size yakawedzera kufanana, SPE column kuyerera mwero wakanyanya kugadzikana, inova classical polymer column murabhoritari, iyo yese nhevedzano yezvigadzirwa yakashandiswa zvakanyanya mukuyedza chikafu.Zvigadzirwa zvakaenzana neMvura 'HLBS, MAX's uye MCX's.

Silica gel matrix akatevedzana SPE

Classic silica gel matrix SPE column, amorphous/spherical adsorbent, ine high cost performance uye yakanaka mushandisi ruzivo nemutengi trust.Zvigadzirwa zvinoshandiswa zvakanyanya mukuongorora makemikari.

Zano kuburitsa/kucheneswa/kuiswa kweSPE

ipfumo rinopinza pombi mudziyo une basa rinoisirwa kuburitsa / kucheneswa kupfumisa biological samples kana iyo Tip yeSPE, chinangwa chigadzirwa chepipetting pfumo kumusoro wedzera imwe C4 / C18 / silicon poda/magnetic beads/ProtingA (G) agarose gel sorbent, inoshandiswa primer/genomic DNA plasmid PCR products/peptide protein/antibodies to target product/filter/extraction/desalting/purification/enrichment.

96/384-gomba ndiro yakatevedzana SPE

Iyo 96/384-well plate series SPE yakagadzirwa zvakanangana nepamusoro-soro sampuli pretreatment.Pedzisa sample pre-processing process nekambani orifice filter kana automatic workstation.

Special yekuona akatevedzana SPE

Azo dhayi yakakosha yekuona koramu: sarudza yekupedzisira-yakachena diatomite filler; Yakasarudzika sieve-ndiro kuyerera chiyero chekudzora tekinoroji, kuburikidza neyemumba uye yekunze ane mvumo masangano ekuyedzwa, OEM kupa.

Pamusoro pezvo, kune graphitized kabhoni dema, acid alkali neutral alumina, coconut shell activated kabhoni, uchi hwekuona column, leather decolorization column, plasticizer inoona column ......SPE column, more SPE products please bvunza.

Matanho (tora Zano SPE semuenzaniso):

Iko kushanda kwakasiyana zvishoma zvichienderana nekuchengetedza nzira ye sorbent ine adsorption yezvinotangwa zvigadzirwa kana tsvina.

1. Iyo sorbent inoshambadza chigadzirwa chakanangwa, inobvisa tsvina uye inonzvenga chigadzirwa chakanangwa

Musimboti:

kurodha sampuli yekuchenesa, kurambwa kusuka, elution

Rudzi urwu rwekusimba kwechikamu chekuwedzera kushanda kazhinji kune matanho mana:

(1) activation - kubviswa kwetsvina muTip SPE uye chizvarwa cheimwe solvent nharaunda;

(2) sampu yekutakura - bvisa sampu mune imwe solvent, shandisa pipette kufemesa Tip SPE uye chengeta zvikamu paTip;

(3) leaching - yakanyanya kubviswa kwetsvina uye zvimwe zvisiri-zvinotangwa zvigadzirwa;

(4) elution - elution uye kuunganidzwa kwechinangwa chechigadzirwa chine vhoriyamu diki yekunyungudutsa.

2. The sorbent adsorbs tsvina uye inobvisa chinangwa chigadzirwa

Musimboti:

Adsorb tsvina pane sampuli uye dzinga iyo yakanangwa chigadzirwa

Rudzi urwu rwekudhonza chikamu chakasimba kazhinji rine matanho matatu:

(1).Activation - bvisa tsvina muTip SPE uye gadzira imwe nzvimbo inonyungudutsa.

(2) kurodha sampuli - inhale Zano SPE nepipette wobva waifuridza zvishoma nezvishoma.Panguva ino, mazhinji emakomisheni anonangwa anozopeperetswa nesample base solution, uye tsvina ichachengetwa paTip.Naizvozvo, matanho anofanira kutanga kuunganidza.

(3) Elution - kuburitsa zvikamu zvine vhoriyamu diki yekunyungudutsa uye kuunganidza, kusanganisa mvura yekuunganidza.

Zvimwe zvinotsanangurwa kana kuti munhu akasarudzika, tinogamuchirwavatengi vese vatsva nevakuru kubvunza, kukurukura kubatana, kutsvaga budiriro yakafanana!