Our Hot-Sale Products Ultrafiltration Centrifugal Tube (Centrifugal Filter)Series Products

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BM Life ScienceUltrafiltration Centrifugal Tube (Centrifugal Filter) Series Products

It consists of an inner filter tube (with membrane) + an outer centrifuge tube, which can quickly and effectively purify and concentrate up to 15ml of biological samples. The unique vertical design and maximized filtration area can provide fast sample processing capacity and high sample recovery rate (usually ≥90% dilute original solution), while maintaining a mild filtration and concentration environment to maintain protein activity and Conformation.The unique vertical design can polarize the solute and significantly reduce the fouling of the filter membrane caused by subsequent centrifugation. By optimizing the physical filter stop point in the filter device, it can prevent the centrifugal filter from drying out the sample or causing sample loss due to excessive rotation. . A variety of ultrafiltration molecular weight cutoff (MWCO) centrifugal filters use high-purity, low-adsorption PES and RCE membranes, which have extremely low protein and nucleic acid binding rates.

BM Life Science, clean room production in all links of Ultrafiltration Centrifugal Tube (Centrifugal Filter), assembly line operation, full ERP management, ultra-pure products, no DNase/RNase, no PCR inhibitors, no heat source. BM Life Science, all sizes of Ultrafiltration Centrifugal Tube (Centrifugal Filter) series products are customized, the series of products are stable in batches with minimal difference between batches and high in quality. They can be replaced by domestic products and exported to Japan, South Korea, Europe, and the United States. Widely used in conventional the sample separation and filtration experiments.

Application

1 Ultrafiltration   Buffer replacement, purification, concentration, and desalination of antigens, antibodies, enzymes, and other proteins, as well as biological samples such as nucleic acids and microorganisms, desalination of chromatographic components, separation of biological molecules from tissue culture or cell lysis solutions, removal of primers, connectors, or molecular markers from reaction mixtures, virus concentration or separation, and crude separation of biological molecule mixtures.

2 Microfiltration   separates nucleic acids and proteins in gels, samples are filtered and clarified, and processed particles or beads are collected and washed.

Feature of Product

★ High concentration ratio, which can easily reach 80-100 times concentration ratio;

 The sample concentration speed is fast, and the general concentration time is 10-60 minutes. It also has an anti-drying lock design to avoid damage to the sample due to excessive centrifugation;

★ The sample recovery rate is high and can reach a recovery rate of more than 90%;

★ Low adsorption, PES/RC membrane and smooth inner wall design have extremely low protein adsorption and biomolecule binding rate;

4ml/15ml、15ml/50ml

★ Complete specifications, ultrafiltration centrifuge tubes with two volumes of 4ml/15ml and 15ml/50ml, with various pore sizes, available in 3KD, 5KD, 10KD, 30KD, 50KD and 100KD to meet different molecular weight cutoff requirements;

★ Convenient operation, the entire separation and filtration process can be completed with a Multifunctional Centrifuge, easy to operate;

★ The product is clean, all links are produced in a clean room, assembly line operation, full ERP management, the product is ultra-pure, no DNase/RNase, no PCR inhibitor, no heat source;

★ High definition, no background and auto-fluorescence, and does not affect the detection results;

★ Reliable performance, good sealing, and does not react with the experimental samples, the experimental results are reliable, and can withstand high temperatures of 100 ℃!

★ It has a wide range of applications. For different applications, two types of Ultrafiltration Centrifugal Tube (Centrifugal Filter) series products, 4ml/15ml and 15ml/50ml are introduced to meet different customer needs, accepting customization and functional development!

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Order Information

Cat.No Name Specification Description Pcs/pk
UCT015RCE004003 Ultrafiltration Centrifugal Tube 4/15ml,3kD ,RCE Membranes 15 pcs/bag
UCT015RCE004010 Ultrafiltration Centrifugal Tube 4/15ml,10kD RCE Membranes 15 pcs/bag
UCT015RCE004030 Ultrafiltration Centrifugal Tube 4/15ml,30kD RCE Membranes 15 pcs/bag
UCT015RCE004100 Ultrafiltration Centrifugal Tube 4/15ml,100kD RCE Membranes 15 pcs/bag
UCT050RCE015003 Ultrafiltration Centrifugal Tube 15/50ml,3kD RCE Membranes 24 pcs/bag
UCT050RCE015010 Ultrafiltration Centrifugal Tube 15/50ml,10kD RCE Membranes 24 pcs/bag
UCT050RCE015030 Ultrafiltration Centrifugal Tube 15/50ml,30kD RCE Membranes 24 pcs/bag
UCT050RCE015100 Ultrafiltration Centrifugal Tube 15/50ml,100kD RCE Membranes 24 pcs/bag
UCT050PES015005 Ultrafiltration Centrifugal Tube 15/50ml,5kD PES Membranes 12 pcs/bag
UCT050PES015010 Ultrafiltration Centrifugal Tube 15/50ml,10kD PES Membranes 12 pcs/bag
UCT050PES015030 Ultrafiltration Centrifugal Tube 15/50ml,30kD PES Membranes 12 pcs/bag
UCT050PES015050 Ultrafiltration Centrifugal Tube 15/50ml,50kD PES Membranes 12 pcs/bag
UCT050PES015100 Ultrafiltration Centrifugal Tube 15/50ml,100kD PES Membranes 12 pcs/bag
CF050PES015C010 Centrifugal Filter 15/50ml,0.1um 0.1um,PES Membranes 12 pcs/bag
CF050PES015C020 Centrifugal Filter 15/50ml,0.2um 0.2um,PES Membranes 12 pcs/bag
CF050PES015C045 Centrifugal Filter 15/50ml,0.45um 0.45um,PES Membranes 12 pcs/bag
UCT** Ultrafiltration Centrifugal Tube Customizing Customizing ** pcs/bag
CF** Centrifugal Filter Customizing Customizing ** pcs/bag

BM Life Sciences, Ultrafiltration Centrifugal Tube (Centrifugal Filter) series product parameters and experimental data

Ultrafiltration Centrifugal Tube (Centrifugal Filter) series product performance and experimental data

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Ultrafiltration Centrifugal of samples:

5 KD 0.25mg/ml   Cyt C;

10 KD1mg/ml     OVA;

30/50 KD 1mg/ml  BSA;  

100 KD 1mg/ml    IgG。  

Experimental conditions : horizontal rotor, centrifugal force 4000×g , 8℃, starting volume 15ml.  

Ultrafiltration Centrifugal of samples:

5 KD 0.25mg/ml   Cyt C;

 10 KD 1mg/ml     OVA;

30/50 KD 1mg/ml  BSA;

100 KD 1mg/ml    IgG.

Experimental conditions : fixed angle rotor, centrifugal force 5000×g , 8℃, starting volume 12ml.

Typical rejection rates for protein molecular weight standards (4000g x 15ml starting volume for rocking rotor or 5000g x 12ml starting volume for fixed angle rotor)

molecular weight standards/concentration molecular weight NMWL Ultrafiltration tube  rejection rates % Set the bucket  Rejection rates % fixed angle  centrifugation time
B-12 vitamin 1350  3K <25 <25 60
(0.25mg/ml)Cytochrome C 12400   >95 >95 60
(1mg/ml)α-Chymotrypsinogen 25000   >95 >95 60
B-12(0.2mg/ml) vitamin 1350  10K <5 <5 30
0.25mg/ml)Cytochrome C 12400   >95 >95 30
(1mg/ml)α-Chymotrypsinogen 25000   >95 >95 30
vitamin B-12(0.2mg/ml) 1350  30K <5 <5 20
Cytochrome C 0.25mg/ml) 12400   <10 <10 20
Ovalbumin(1mg/ml) 45000   >95 >95 20
BSA(1mg/ml) 67000   >95 >95 20
Cytochrome C(0.25mg/ml) 12400  50K <5 <5 10
Ovalbumin(1mg/ml) 45000   65 55 10
BSA(1mg/ml) 67000   >90 >90 10
Ovalbumin(1mg/ml 45000  100K <25 <15 20
IgG(1mg/ml) 156000   >90 >90 20
Thyroglobulin(0.5mg/ml) 677000   >90 >90 20

Recovery rate

The retention capacity of the 15ml ultrafiltration centrifuge tube membrane is described by the molecular weight cutoff (MWCO).

Solute molecular weights close to MWCO may only be partially retained. In order to obtain the best retention characteristics,         

please use a MWCO filter membrane with a molecular weight of 1/3 or less than the solute.

Product recovery rate comparison

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Comparison of protein recovery rates and dead volumes of different brands of ultrafiltration tubes

10KD Comparison of protein recovery rates and dead

volumes of different brands of ultrafiltration tubes

Brand (%)Recovery rate (μl)Dead volume
BM 1㎎/ml  Protein concentration 90 87(Protein concentration1㎎/ml)
0.1㎎/ml Protein concentration 83  
Brand M 1㎎/ml Protein concentration 93 47(Protein concentration1㎎/ml)
  0.1㎎/ml Protein concentration 88  
Brand S 1㎎/ml Protein concentration 91 70(Protein concentration1㎎/ml)
  0.1㎎/ml Protein concentration 85  
Brand K 1㎎/ml Protein concentration 89 186(Protein concentration1㎎/ml)
  0.1㎎/ml Protein concentration 77  
Brand J 1㎎/ml Protein concentration 83  
  0.1㎎/ml Protein concentration 50  
  Experimental conditions: OVA protein (molecular weight 45KD), horizontal rotor 4000g
 

Reference selection specifications for ultrafiltration tubes with different molecular weights

  starting volume <0.5ml <2ml <4ml <15ml
Protein
(MW)molecular weight   MWCO (Da) molecular weight cutoff      
  6K<MW<20K 3000 3000 3000 3000
  20K<MW<60K 10000 10000 10000 10000
  60K<MW<100K 30000 30000 30000 30000
  100K<MW<200K 50000 50000 50000 50000
  200K<MW 100000 100000 100000 100000
Single-stranded and double-stranded nucleic acids
length   MWCO (Da) molecular weight cutoff      
  137-1159bp 3000 3000 3000 3000
nanoparticles
(DIA)particle size   MWCO (Da) molecular weight cutoff      
  1.5<DIA<3nm 3000 3000 3000 3000
  3<DIA<5nm 10000 10000 10000 10000
  5<DIA<7nm 30000 30000 30000 30000
  7<DIA<10nm 50000 50000 50000 50000
  10 nm<DIA 100000 100000 100000 100000

Post time: Feb-18-2024