I-SPME inezisekelo ezintathuukukhishwaizindlela: Direct Ectraction SPME, Headspace SPME kanye ne-membrane-protected SPME.

1) Isizinda esiqondile

Ngendlela eqondile yokukhipha, i-quartz fiber ehlanganiswe neukukhishwaisigaba esimile sifakwa ngokuqondile ku-matrix yesampula, futhi izingxenye eziqondiwe zidluliswa ngokuqondile zisuka ku-matrix yesampula ziye esigabeni esimile sokukhipha.Ngesikhathi sokusebenza kwaselabhorethri, izindlela zokuyaluza zivame ukusetshenziselwa ukusheshisa ukusakazeka kwezingxenye zokuhlaziya ukusuka ku-matrix yesampula kuya onqenqemeni lwesigaba esimile sokukhipha.Kumasampula egesi, ukuthuthwa kwegesi kwemvelo kwanele ukusheshisa ukulingana kwezingxenye zokuhlaziya phakathi kwezigaba ezimbili.Kodwa kumasampula amanzi, isivinini sokusabalalisa sezingxenye emanzini singama-oda angu-3-4 wobukhulu obuphansi kunalawo amagesi, ngakho-ke ubuchwepheshe bokuxuba obusebenzayo buyadingeka ukuze kuzuzwe ukusakazeka okusheshayo kwezingxenye kusampula.Izindlela zokuxuba ezivame ukusetshenziswa kakhulu zihlanganisa: ukusheshisa izinga lokugeleza kwesampula, ukunyakazisa ikhanda le-fiber ekhishwe noma isitsha sesampula, ukugqugquzela i-rotor kanye ne-ultrasound.

Ngakolunye uhlangothi, lezi zindlela zokuxuba zisheshisa izinga lokusabalalisa lezingxenye ku-matrix yesampula yevolumu enkulu, futhi ngakolunye uhlangothi, zinciphisa lokho okubizwa ngokuthi "indawo yokulahlekelwa" okubangelwa ungqimba lwe-sheath yokuvikela yefilimu ewuketshezi eyakhelwe phezu. udonga lwangaphandle lwesigaba esimile sokukhipha.

2) Ukukhishwa kwe-Headspace

Kumodi yokukhipha i-headspace, inqubo yokukhipha ingahlukaniswa ngezinyathelo ezimbili:
1. Ingxenye ehlaziywe iyahlakazeka futhi ingene isuka esigabeni esiwuketshezi iye esigabeni segesi;
2. Ingxenye ehlaziywe idluliswa isuka esigabeni segesi iye esigabeni esimile sokukhipha.
Lokhu kuguqulwa kungavimbela isigaba esimile sokukhipha ekungcolisweni izinto ezinamamolekyuli aphezulu nezinto ezingaguquguquki kumasampula athile ama-matrices (njengokuphuma komuntu noma umchamo).Kule nqubo yokukhipha, isivinini sokukhipha sesinyathelo sesi-2 ngokuvamile sikhulu kakhulu kunejubane lokusabalalisa lesinyathelo 1, ngakho isinyathelo soku-1 siba isinyathelo sokulawula sokukhipha.Ngakho-ke, izingxenye eziguquguqukayo zinezinga lokukhishwa elishesha kakhulu kunezingxenye eziguquguquka kancane.Eqinisweni, ezingxenyeni eziguquguqukayo, ngaphansi kwezimo ezifanayo zokuxuba isampula, isikhathi sokulinganisa sokukhishwa kwe-headspace sifushane kakhulu kuneso sokukhishwa okuqondile.

3) Ukukhishwa kokuvikela ama-membrane

Inhloso enkulu yokuvikela ulwelwesi lwe-SPME ukuvikelaukukhishwaisigaba esimile emonakalweni lapho kuhlaziywa amasampula angcole kakhulu.Uma kuqhathaniswa ne-SPME yokukhipha i-headspace, le ndlela inenzuzo kakhulu ekukhishweni nasekuthuthukisweni kwezingxenye eziqinile ukuze ziguquke.Ngaphezu kwalokho, ifilimu yokuzivikela eyenziwe ngezinto ezikhethekile inikeza izinga elithile lokukhetha inqubo yokukhipha.