Usoro izugbe maka mmịpụta akụkụ siri ike bụ nke a:

1. Na-arụ ọrụ na adsorbent: Jiri ihe mgbaze kwesịrị ekwesị kpochaa ihe siri ike na-adọpụ oge tupu ị wepụ ihe nlele ahụ iji mee ka adsorbent dị mmiri, nke nwere ike ịkwado ogige ndị e lekwasịrị anya ma ọ bụ ihe na-egbochi ya.Ụdị dị iche iche nke ịgbalite katrijị mmịpụta siri ike na-eji ihe mgbaze dị iche iche:

(1) Adsorbents ndị na-adịghị ike ma ọ bụ ndị na-abụghị polar ndị a na-eji na-agbanwe agbanwe-phase siri ike mmịpụta na-ejikarị ihe mgbaze organic na-agbaze mmiri, dị ka methanol, wee kpochaa ya na mmiri ma ọ bụ ihe ngwọta.Ọ ga-ekwe omume ịsacha ya na ihe mgbaze siri ike (dị ka hexane) tupu ịsacha ya na methanol iji kpochapụ adịghị ọcha na-agbasa na adsorbent na nnyonye anya ha na ogige ebumnuche.

(2) Adsorbent polar a na-eji na mmịpụta ihe siri ike na-emekarị na-ejikarị ihe mgbaze organic (sample matrix) ebe ogige ebumnuche dị.

(3) The adsorbent eji na ion-exchange siri ike oge mmịpụta nwere ike ịsacha na sample ihe mgbaze mgbe a na-eji maka samples na-abụghị polar organic solvents;mgbe a na-eji ya maka ihe nlele na polar solvents, enwere ike ịsacha ya na mmiri na-edozi mmiri mgbe ịsachara, kpochaa ya na ngwọta mmiri nke uru pH kwesịrị ekwesị ma nwee ụfọdụ ihe mgbaze na nnu.

Iji mee ka sorbent dị na katrij SPE dị mmiri mgbe emechara ya na tupu mgbakwunye ihe atụ, ihe dị ka 1 ml nke ihe mgbaze maka ịgbalite kwesịrị idowe ya na sorbent mgbe emechara ya.

2. Nlereanya nlele: Wunye mmiri mmiri ma ọ bụ gbazere ihe nlele siri ike n'ime katrijị mmịpụta siri ike na-arụ ọrụ, wee jiri oghere, nrụgide ma ọ bụ centrifugation mee ka nlele ahụ banye adsorbent.

3. Ịsacha na elution: Mgbe ihe nlele ahụ abanyela na adsorbent na ihe a na-atụ anya ya, a pụrụ iji ihe mgbaze na-esighị ike na-asachapụ nke na-adịghị ike, mgbe ahụ, a ga-eji ihe mgbaze siri ike mee ihe na-ekpo ọkụ ma kpokọta ya..Rinse na Elution Dị ka akọwara na mbụ, eluent ma ọ bụ eluent nwere ike ịfefe na adsorbent site na oghere, nrụgide ma ọ bụ centrifugation.

Ọ bụrụ na a na-ahọrọ adsorbent ka ọ bụrụ nke na-adịghị ike ma ọ bụ na-enweghị mgbasa ozi na ihe a na-atụ anya ya na mgbasa ozi siri ike na ntinye aka na-etinye aka, a pụkwara ịsacha ihe ahụ ma na-anakọta ya na mbụ, ebe a na-ejigide ihe ntinye aka (adsorption).) na adsorbent, a na-ekewa ha abụọ.N'ọtụtụ ọnọdụ, a na-edobe ihe ezubere iche na adsorbent, na n'ikpeazụ, a na-eji ihe mgbaze siri ike mee ihe, nke na-eme ka ọ dị ọcha nke ihe nlele ahụ.