Njira yayikulu yochotsera gawo lolimba ndi motere:

1. Kuyambitsa adsorbent: Muzimutsuka cholimba gawo m'zigawo katiriji ndi zosungunulira yoyenera musanatenge chitsanzo kusunga adsorbent yonyowa, amene angathe adsorb chandamale mankhwala kapena kusokoneza mankhwala.Njira zosiyanasiyana zoyatsira makatiriji olimba a gawo lolimba amagwiritsa ntchito zosungunulira zosiyanasiyana:

(1) Ofooka polar kapena non-polar adsorbents ntchito reversed-gawo olimba-gawo m'zigawo nthawi zambiri rins ndi madzi sungunuka zosungunulira organic, monga methanol, ndiyeno nachapidwa ndi madzi kapena bafa njira.Komanso n'zotheka muzimutsuka ndi zosungunulira amphamvu (monga hexane) pamaso rinsing ndi methanol kuthetsa zosafunika adsorbent pa adsorbent ndi kusokoneza chandamale pawiri.

(2) The polar adsorbent ntchito yachibadwa-gawo olimba-gawo m'zigawo zambiri eluted ndi organic zosungunulira (chitsanzo masanjidwewo) kumene chandamale pawiri ili.

(3) The adsorbent ntchito ion-kusinthanitsa olimba gawo m'zigawo akhoza kutsukidwa ndi zosungunulira chitsanzo pamene ntchito zitsanzo mu sanali polar organic solvents;ikagwiritsidwa ntchito ngati zitsanzo mu zosungunulira za polar, zimatha kutsukidwa ndi zosungunulira zamadzi zosungunuka m'madzi Mukatsukidwa, muzimutsuka ndi njira yamadzi ya pH yoyenera komanso yokhala ndi zosungunulira ndi mchere.

Kuti sorbent mu SPE katiriji yonyowa pambuyo kutsegula ndi pamaso chitsanzo Kuwonjezera, za 1 ml ya zosungunulira kuti kutsegula ayenera kusungidwa pa sorbent pambuyo kutsegula.

2. Zitsanzo Mumakonda: Thirani madzi kapena kusungunuka olimba chitsanzo mu adamulowetsa olimba gawo m'zigawo katiriji, ndiyeno ntchito zingalowe, mavuto kapena centrifugation kupanga chitsanzo kulowa adsorbent.

3. Kutsuka ndi elution: Pambuyo chitsanzo akulowa adsorbent ndi chandamale pawiri ndi adsorbed, ndi ofooka anapitiriza kusokoneza pawiri akhoza kutsukidwa ndi zosungunulira ofooka, ndiyeno chandamale pawiri akhoza eluted ndi zosungunulira wamphamvu ndi kusonkhanitsa..Muzimutsuka ndi Elution Monga tafotokozera kale, loents kapena loent akhoza kudutsa adsorbent ndi vacuum, kuthamanga kapena centrifugation.

Ngati adsorbent imasankhidwa kuti ikhale yofooka kapena yopanda kulengeza ku gulu lachindunji ndi kulengeza mwamphamvu kwa gulu losokoneza, gulu lachindunji lingathenso kutsukidwa ndikusonkhanitsidwa poyamba, pamene chigawo chosokoneza chimasungidwa (adsorption).) pa adsorbent, awiriwa amalekanitsidwa.Nthawi zambiri, chandamale pawiri anakhalabe pa adsorbent, ndipo potsiriza eluted ndi zosungunulira amphamvu, amene ndi yabwino kuyeretsedwa kwa chitsanzo.