Ko te tikanga whanui mo te tangohanga wahanga totoka e whai ake nei:

1. Whakahohe i te whakangao: Horoihia te kaata tangohanga wahanga totoka me te whakarewa tika i mua i te tangohanga i te tauira kia makuku te whakangao, ka taea te whakauru i nga puhui whainga, i nga puhui pokanoa ranei.He rereke nga momo whakarewatanga o te kariri tangohanga wahanga totoka e whakamahi ana i nga whakarewa rereke:

(1) Ko nga mea ngoikore o te polar, kore-polar adsorbents ranei e whakamahia ana i roto i te tangohanga totika-waahanga whakamuri ka horoia ki te whakarewa pararopi wairewa-wai, penei i te methanol, katahi ka horoia ki te wai, ki te otinga parepare ranei.Ka taea hoki te horoi ki te whakarewa kaha (pēnei i te hexane) i mua i te horoi ki te methanol hei whakakore i nga poke i uru ki runga i te whakangao me te pokanoa ki te pūhui i whäia.

(2) Ko te whakangao polar e whakamahia ana i roto i te tangohanga-waa-waa-waa-waahanga-a-waa-a-waa ka whakahekehia ki te whakarewa pararopi (tauira matrix) kei reira te puhui whaainga.

(3) Ko te adsorbent e whakamahia ana i roto i te tangohanga totoka whakawhiti katote ka taea te horoi me te tauira whakarewa ka whakamahia mo nga tauira i roto i nga whakarewa pararopi kore-polar;ina whakamahia ana mo nga tauira i roto i nga whakarewa polar, ka taea te horoi ki nga whakarewa pararopi wairewa-wai I muri i te horoinga, horoi ki te wairewa wai o te uara pH tika me etahi whakarewa pararopi me nga tote kei roto.

Kia mau tonu te makuku i roto i te kariri SPE i muri i te whakahohenga me mua i te taapiri tauira, kia mau ki te 1 ml o te whakarewa mo te whakahohenga ki runga i te sorbent i muri i te whakahohenga.

2. Tauira uta: Ringihia te wai, te tauira totoka rewa ranei ki roto i te kaata tangohanga wahanga totoka whakahohe, katahi ka whakamahi i te korehau, te pehanga, te centrifugation ranei kia uru te tauira ki roto i te adsorbent.

3. Te horoi me te whakahiato: I muri i te urunga o te tauira ki roto i te adsorbent me te whakauru i te puhui whaainga, ka taea te horoi i te puhui pokanoa e mau tonu ana me te whakarewa ngoikore, katahi ka taea te whakakore i te kohinga whaainga me te whakarewa kaha ake ka kohia..Horoi me te Elution Ka rite ki te korero i mua ake nei, ka taea te whakawhiti te eluent, te eluent ranei i roto i te adsorbent ma te korehau, te pehanga, te centrifugation ranei.

Mena ka tohua te adsorbent kia ngoikore, kore ranei he adsorption ki te puhui whaanui me te kaha o te whakauru ki te puhui pokanoa, ka taea hoki te horoi me te kohi tuatahi te puhui whaanui, ka mau tonu te puhui pokanoa (adsorption).) i runga i te adsorbent, ka wehea nga mea e rua.I roto i te nuinga o nga wa, ka mau tonu te puhui i runga i te adsorbent, ka mutu ka whakahekehia me te whakarewa kaha, he pai ake ki te purenga o te tauira.