Nc Membrane And Protein Transfer Membrane

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NC/PVDF Transfer Film Series Consumables Products

The series of transfer films have high binding capacity (electrostatic, hydrophobic) to proteins, providing excellent sensitivity and low background, and enabling multiple immunoassays.

BM Life Sciences' NC/PVDF transfer membranes are tailored for specific applications, assays and blotting techniques in biopharmaceuticals, medicine and other fields with excellent performance.

It has the advantages of high strength, repeatable peeling, high chemical compatibility, and uniform and stable structure.

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Product Features

* Imported medical-grade raw materials, the products are highly transparent and ultra-pure are highly transparent and ultra-pure;
* The product is not easy to crack or break when cutting, and the unique surface structure and properties create an excellent signal-to-noise ratio;
* The raw material for NC transfer films is derived from natural fibers, is environmentally friendly, has high chemical compatibility, does not require pre-activation and keeps the bound proteins active for a longer period of time;
* Multiple regeneration testing is possible, and sterile products can be supplied according to the different needs of customers;
* Various products and specifications meet the different needs of different customers;

0.1um For proteins with molecular weight less than 7KDa;

0.22um for proteins with molecular weight less than 20KDa;

0.45um is suitable for proteins with molecular weight greater than 20KDa;
* Applicable to a variety of detection methods: chemiluminescence, fluorescence detection, enzyme-linked antibodies, direct staining, radiolabeled probes;
* Clean room production in all links of the product, assembly line operation, optical robot quality inspection, full ERP management;
* Wide range of applications: Suitable for Western Blotting (WB), Southern blotting, Northern blotting, dot/narrow line hybridization analysis, mass spectrometry amino acid analysis, N-terminal protein sequencing, protein visualization and other experiments, this series of products to accept customer customization and function development!

Electron Microscope Microstructure of PVDF Transfer Membrane

PVDF-01 PVDF-02 PVDF-03

Surface electron micrograph of Electon microscopy of upper surface of Electron microscopy of undersurface of
0.22um PVDF transfer film 0.45um PVDF transfer film 0.45um PVDF transfer film

Surface electron microscopy showed that the PVDF transfer membrane had a compact and homogeneous pore structure, which had a high internal surface area and a high and homogeneous protein absorption load.

WB Experimental Results Graph

Experimental parameters:

Target proteins: E. coli broken bacterial supernatant protein (left), bovine serum albumin (right)

Wash solution: TBST

Closure: Closed with 5% skimmed milk powder shaker at room temperature with slow shaking for about 1 h.

Primary antibody: rabbit anti-E. coli antibody, rabbit anti-BSA polyclonal antibody

Secondary antibody: dilute the secondary antibody with TBST at the ratio of 1:10,000 and incubate for about 1h at room temperature on a shaker.

Developing: Mix ECL chemiluminescent solution A and solution B according to 1:1, and add them evenly onto the membrane, and then analyze by exposure with Fusion imaging system.

Membrane transfer conditions: constant current 400 mA, wet transfer for 40 min.

result result 2

WB Comparison Tests Show Excellent Protein Transfer Properties of PVDF Transfer Films from Biomax Life Sciences

Order Details

Product Number	Name	Description	Filter Size	Pcs/Pk	Unit	Pcs/WCN
MMNCT010101	NC transfer membrane	Pore:0.1μm, Thickness:145um, Applicable proteins＜10KDa	100*100mm	25 pcs/bag	case	100
MMNCT010202		Pore:0.1μm, Thickness:145um, Applicable proteins＜10KDa	150*150mm	25 pcs/bag	case	100
MMNCT010330		Pore:0.1μm, Thickness:145um, Applicable proteins＜10KDa	300mm*3m	1 roll/ bag	case	10
MMNCT010340		Pore:0.1μm, Thickness:145um, Applicable proteins＜10KDa	300mm*4m	1 roll/ bag	case	10
MMNCT020101		Pore:0.22μm, Thickness:145um, Applicable proteins10-20KDa	100*100mm	25 pcs/bag	case	100
MMNCT020202		Pore:0.22μm, Thickness:145um, Applicable proteins10-20KDa	150*150mm	25 pcs/bag	case	100
MMNCT020330		Pore:0.22μm, Thickness:145um, Applicable proteins1-200KDa	300mm*3m	1 roll/ bag	case	10
MMNCT020340		Pore:0.22μm, Thickness:145um, Applicable proteins10-20KDa	300mm*4m	1 roll/ bag	case	10
MMNCT040101		Pore:0.45μm, Thickness:145um, Applicable proteins＞20KDa	100*100mm	25 pcs/bag	case	100
MMNCT040202		Pore:0.45μm, Thickness:145um, Applicable proteins＞20KDa	150*150mm	25 pcs/bag	case	100
MMNCT040330		Pore:0.45μm, Thickness:145um, Applicable proteins＞20KDa	300mm*3m	1 roll/ bag	case	10
MMNCT040340		Pore:0.45μm, Thickness:145um, Applicable proteins＞20KDa	300mm*4m	1 roll/ bag	case	10
MMPVT020101	PVDF transfer membrane	Pore: 0.22μm, protein binding capacity＞300μg/cm2 ,(BSA)	100*100mm	25 pcs/ bag	case
MMPVT020202	PVDF transfer membrane	Pore: 0.22μm, protein binding capacity＞300μg/cm2 ,(BSA)	150*150mm	25 pcs/ bag	case
MMPVT020330	PVDF transfer membrane	Pore: 0.22μm, protein binding capacity＞300μg/cm2 ,(BSA)	300*3000mm	1 roll/ bag	case
MMPVT040101	PVDF transfer membrane	Pore: 0.45μm, protein binding capacity＞200μg/cm2 (BSA)	100*100mm	25 pcs/ bag	case
MMPVT040202	PVDF transfer membrane	Pore: 0.45μm, protein binding capacity＞200μg/cm2 (BSA)	150*150mm	25 pcs/ bag	case	1250
MMPVT040330	PVDF transfer membrane	Pore: 0.45μm, protein binding capacity＞200μg/cm2 (BSA)	300*3000mm	1 roll/ bag	case	1250
MM 00*	membrane & transfer membrane	Personalized Customization		Customizing	case	Customizing