Please leave a message
Многофункциональная колонка для очистки афлатоксина

Technical Data

Многофункциональная колонка для очистки афлатоксина

● Aflatoxins (AFLs) are a group of toxic metabolites produced by various mold fungi such as Aspergillus flavus and Aspergillus parasiticus with a similar chemical structure, Aspergillus terreus also produces aflatoxins but with a lower yield and it is the most common class.
● It is the most common type of mycotoxins, 12 types have been isolated and identified, among which the main molecular types are: B1, B2, G1,
● G2, M1, M2 and so on. They are usually found in moldy cereals and oilseeds, their processed products and feed.
● It is a carcinogen, toxic to the human body and animal organs, belongs to class 1 carcinogens.   
● Aflatoxin Purification Column BMAFT226 contains multiple adsorbent substrates, which can quickly and selectively adsorb aflatoxin in the sample,
● Selective adsorption of lipids, proteins, pigments and other impurities in the sample, and does not adsorb the components to be measured to achieve rapid purification of aflatoxin.
● It can quickly purify aflatoxin compounds G, B, and M without adsorbing the components being measured.
● At the same time, it can be combined with LC-MS/MS or HPLC to achieve the purpose of rapid detection and accurate quantification.
● The method can be used in combination with LC-MS/MS or HPLC instruments to achieve rapid detection and accurate quantification.
image001t6c
image003jqy

Product Specifications

★ Medical grade imported raw materials production, the product is highly transparent, ultra-pure;
★ Diversified products, different specifications to meet the different needs of different customers; 
★ Simple and fast, 30 seconds can complete the cleaning; long shelf life, room temperature can be stored for 24 months; Recovery rate: ≥ 90%, RSD ≤ 5%; Applicable to GB5009.185-2016, GB5009.22-2016 and other national standards;
★ All parts of the product clean room production, assembly line operation, optical quality control robot, all ERP management, standardization of the production process, product quality can be traced;
★ Wide Range of Applications: Suitable for various aflatoxin detection experiments, a series of products to adopt customer personalization and functional development!

Use

Remove the sample, add the extract to the tube, insert the end of the rubber-tipped cleaning column into the tube, press the bottom of the tube, the purified liquid will reach the top of the cleaning column through the package, pour out the liquid, dry and re-dissolve the liquid, then it can be tested on the machine.
For more details, please refer to the instruction manual.

Order Details

Product Number

Product Name

Description

wrap unit
BMAFT226

Multifunctional column for aflatoxin purification

5 мл/бранка, 25 наборов/коробка (включая пробирки)

25

box

AFT-IACT101

Total Aflatoxin Columns

1ml/pc, 25pcs/carton

25

box

AFT-IACT103 Total Aflatoxin Columns

5ml/pcs, 20pcs/carton

20

box

AFT-IACB101 Aflatoxin B1 Columns

5ml/pc, 25pcs/carton

25

box

AFT-IACB103 Aflatoxin B1 Columns

3ml/pcs, 20pcs/carton

20

box

AFT-IACM101 Aflatoxin M1 Columns

1ml/pc, 25pcs/carton

25

box

AFT-IACM103 Aflatoxin M1 Columns

3ml/pcs, 20pcs/carton

20

box

AFT- 00* Aflatoxin tubes/plates

Personalization

   

user’s guide

The multifunctional BMAFT226 purification column allows the detection of aflatoxin compounds G, B, M, by LC-MS/MS or HPLC, quantitatively and accurately.

Sample Type

Sample Quality

Liquid Extract

Extraction Method

Peanut oil and its vegetable oils, etc.

5g

20ml 84% Acetonitrile/Water

High-speed homogenization (≥10,000 rpm) for 5 minutes, vigorous shaking (200-300 rpm) for 20 minutes, or ultrasonic extraction for 30 minutes, the filtrate was filtered and collected with filter paper for rapid quantitative analysis or placed in a centrifuge (≥7000 rpm for 20 minutes) to collect the supernatant.

Peanuts and their by-products, nuts, flour, wheat and other food crops

20ml 90% acetonitrile/water

Corn meal and feed

20ml 95% acetonitrile/water

Chinese medicines (19 Chinese medicines listed in the 2015 Pharmacopoeia edition)

20ml 95% Acetonitrile/Water or Pure Acetonitrile

Soy sauce and vinegar

Bring the volume to 25 ml of pure acetonitrile

Sample Cleaning

Step 1: Add 10 mL of sample extract to the test tube, then add 50 mL of acetic acid.
Step 2: Insert the rubber tip 228 of the cleaning column from the top of the tube into the tube and press the column against the bottom of the tube Step 3: Pour the purified sample extract from the top of the column into the sample bottle or EP tube.
Step 4: Take 5ml of purified extract, add 20ml of acetic acid, blow out with nitrogen until dry, use 1ml or 0.5ml of acetic acid solution (10ml of acetic acid per 250ml of ultrapure water) to clean the sample.
Dissolve in 1 ml or 0.5 ml of acetic acid solution (add 10 ml of acetic acid to 250 ml of ultrapure water), pass through a microporous filter membrane, then analyze on the device.
image0017n4
Технологическая схема использования многофункциональной очистительной колонки

Calculation of results

Re-dissolution volume (mL)

Calculated concentration of standardized formula (c, ng/mL)

Sample content(X,μg/kg)

1 c1

X=2c1

0.5 c2

X=c2