ʻO ka Oligonucleotide (Oligonucleotide), e pili ana i kahi ʻāpana polynucleotide linear o 2-10 mau koena nucleotide i hoʻopili ʻia e nā kaula phosphodiester, akā ke hoʻohana ʻia kēia huaʻōlelo, ʻo ka helu o nā koena nucleotide ʻAʻohe kānāwai koʻikoʻi.I loko o nā puke he nui, ua kapa ʻia nā molekole polynucleotide i loaʻa he 30 a ʻoi aku ke koena nucleotide o oligonucleotides.Hiki ke hoʻopili ʻia nā oligonucleotides e nā mea kani.Hiki ke hoʻohana ʻia e like me ka DNA synthesis primers, gene probes, etc., a he ākea ka hoʻohana ʻana i ka noiʻi biology molecular hou.

palapala noi

Hoʻohana pinepine ʻia nā oligonucleotides ma ke ʻano he ʻimi no ka hoʻoholo ʻana i ke ʻano o ka DNA a i ʻole RNA, a hoʻohana ʻia i nā kaʻina hana e like me ka gene chip, electrophoresis, a me ka fluorescence in situ hybridization.

Hiki ke hoʻohana ʻia ka DNA synthesized e ka oligonucleotide i ke kaulahao polymerization reaction, hiki ke hoʻonui a hōʻoia i nā ʻāpana DNA āpau.Ma kēia kaʻina hana, hoʻohana ʻia ka oligonucleotide ma ke ʻano he kumu mua e hui pū me ka ʻāpana hoʻohui i kapa ʻia i loko o ka DNA e hana i kope DNA..

Hoʻohana ʻia nā oligonucleotides hoʻoponopono e kāohi i nā ʻāpana RNA a pale iā lākou mai ka unuhi ʻia ʻana i loko o nā proteins, hiki ke pāʻani i kekahi hana i ka hoʻōki ʻana i ka hana o nā maʻi kanesa.