I-Oligonucleotide (i-Oligonucleotide), ngokuvamile ibhekisela kucezu lwe-polynucleotide olulayini lwezinsalela ze-nucleotide ezingu-2-10 ezixhunywe amabhondi e-phosphodiester, kodwa uma leli gama lisetshenziswa, inani lezinsalela ze-nucleotide Ayikho imithetho eqinile.Ezincwadini eziningi, ama-molecule e-polynucleotide aqukethe izinsalela ze-nucleotide ezingu-30 noma ngaphezulu abizwa ngokuthi ama-oligonucleotides.Ama-oligonucleotides angahlanganiswa ngokuzenzakalelayo ngamathuluzi.Angasetshenziswa njengeziqalo ze-DNA synthesis, ama-gene probe, njll., futhi abe nohlu olubanzi lokusetshenziswa ocwaningweni lwanamuhla lwebhayoloji yamangqamuzana.

isicelo

Ama-oligonucleotides avame ukusetshenziswa njengama-probes ukuze anqume ukwakheka kwe-DNA noma i-RNA, futhi asetshenziswa ezinqubweni ezifana ne-gene chip, i-electrophoresis, ne-fluorescence in situ hybridization.

I-DNA ehlanganiswe yi-oligonucleotide ingasetshenziswa ekuphenduleni kwe-polymerization yeketanga, engakhulisa futhi iqinisekise cishe zonke izingcezu ze-DNA.Kule nqubo, i-oligonucleotide isetshenziswa njenge-primer ukuze ihlanganiswe nesiqephu esihambisanayo esibhalwe ku-DNA ukwenza ikhophi ye-DNA..

Ama-oligonucleotide alawulayo asetshenziselwa ukuvimbela izingcezu ze-RNA futhi zivimbele ukuthi zingahunyushwa zibe amaprotheni, angadlala indima ethile ekumiseni umsebenzi wamangqamuzana omdlavuza.