Oligonucleotide (Oligonucleotide), ka kakaretso e bua ka sekhechana sa polynucleotide se nang le mela ea 2-10 ea masala a nucleotide a kopantsoeng ke litlamo tsa phosphodiester, empa ha lentsoe lena le sebelisoa, palo ea masala a nucleotide Ha ho na melao e thata.Lingoliloeng tse ngata, limolek'hule tsa polynucleotide tse nang le masala a 30 kapa ho feta a nucleotide li boetse li bitsoa oligonucleotides.Oligonucleotides e ka etsoa ka bo eona ka lisebelisoa.Li ka sebelisoa e le DNA synthesis primers, gene probes, joalo-joalo, 'me li na le mefuta e mengata ea tšebeliso lipatlisisong tsa sejoale-joale tsa baeloji ea limolek'hule.

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Hangata li-oligonucleotides li sebelisoa e le li-probes ho fumana sebopeho sa DNA kapa RNA, 'me li sebelisoa lits'ebetsong tse kang gene chip, electrophoresis, le fluorescence in situ hybridization.

DNA e entsoeng ke oligonucleotide e ka sebelisoa ho ketane ea polymerization reaction, e ka holisang le ho tiisa hoo e batlang e le likaroloana tsohle tsa DNA.Ts'ebetsong ena, oligonucleotide e sebelisoa e le primer ho kopanya le sekhechana se tlatselletsang se ngotsoeng ho DNA ho etsa kopi ea DNA..

Li-oligonucleotide tse laolang li sebelisetsoa ho thibela likhechana tsa RNA le ho li thibela ho fetoleloa ho liprotheine, tse ka bapalang karolo e itseng ho emisa mosebetsi oa lisele tsa mofetše.