Ko te wehenga me te purenga o nga pūmua e whakamahia nuitia ana i roto i te rangahau matū koiora me te tono, he pukenga whakahaere nui.Ko SCG Protein Purification System Company-Saipu Instrument kua whakahiato te wehewehenga maru me te ihirangi wehenga pai opūmuapurenga mo te katoa.He maha nga momo pūmua rereke kei roto i tetahi pūtau eukaryotic, he tino taonga etahi, he ruarua noa nga kape kei roto.Hei ako i tetahi pūmua, me pure tuatahi te pūmua mai i etahi atu pūmua me te ngota ngota pūmua kore.

Te wehenga nui

Ina whiwhihia te tangohanga pūmua (i etahi wa ka konatunatua ki te waikawa nucleic, polysaccharides, me etahi atu), ka tohua he huinga tikanga e tika ana hei wehe i te hiahia.pūmuamai i etahi atu poke.Ko te tikanga, ka whakamahia e tenei waahanga wehewehe nga tikanga penei i te tote, te whakaemi ira irahiko me te hautanga whakarewa waro.Ko enei tikanga e tohuhia ana e te ngawari me te kaha o te tukatuka nui, ka taea te tango i nga poke maha me te aro ki te otinga pūmua.Ko etahi tangohanga pūmua he nui te rōrahi, ā, kāore e pai mō te kukū mā te whakaemi, mā te tote rānei.Ka taea e koe te whiriwhiri i te ultrafiltration, te filtration gel, te whakamaroke korehau mīti me etahi atu tikanga mo te kukū.

Te wehenga pai

I muri i te wehenga taratara o te tauira, he iti noa te rōrahi, kua tangohia te nuinga o ngā poke.Mo te purenga ake, ko nga tikanga chromatography te tikanga ko te filtration gel, te chromatography whakawhiti katote, te chromatography adsorption, me te chromatography affinity.Mena e tika ana, ka taea hoki e koe te whiriwhiri i te electrophoresis, tae atu ki te electrophoresis rohe, te huinga tohu isoelectric, me etahi atu hei tukanga purenga whakamutunga.Ko te tikanga e whakamahia ana mo te wehewehe i te taumata wehewehenga he iti noa i roto i te whakamahere, engari he teitei te taumira.

Ko te whakatioatanga te tukanga whakamutunga o te wehenga pūmua me te purenga.Ahakoa karekau te tukanga whakatioatanga e whakarite kia noho rite te pūmua, katahi ka whai painga tetahi pūmua ki te otinga hei hanga karaihe.Ko te tukanga whakatioatanga ano e haere tahi ana me tetahi tohu purenga, a ka taea e te whakamaaramatanga te tango i te iti o te pūmua poke.Mai i te mea kua denaturedpūmuakare ano i kitea i te wa o te whakamaaramatanga, ko te whakamaarama pūmua ehara i te tohu mo te ma, engari he aratohu kaha ki te whakatau ko te hua kei roto i tona ahuatanga.