Ukuhlukaniswa nokuhlanzwa kwamaprotheni kusetshenziswa kakhulu ocwaningweni nasekusetshenzisweni kwe-biochemistry futhi kuyikhono elibalulekile lokusebenza.I-SCG Protein Purification System Company-Saipu Instrument ihlanganise ukuhlukaniswa okungafanele nokuqukethwe okuhle kokuhlukanisaamaprotheniukuhlanzwa kwawo wonke umuntu.Iseli elijwayelekile le-eukaryotic lingaqukatha izinkulungwane zamaphrotheni ahlukene, amanye acebe kakhulu kanti amanye aqukethe amakhophi ambalwa kuphela.Ukuze ufunde iphrotheni ethile, kuyadingeka kuqala ukuhlanza amaprotheni kwamanye amaprotheni nama-molecule angewona amaprotheni.

Ukuhlukana okuqinile

Lapho ukukhishwa kwamaprotheni (ngezinye izikhathi kuhlanganiswe nama-nucleic acid, ama-polysaccharides, njll.) kutholakala, isethi yezindlela ezifanele ziyakhethwa ukuze kuhlukaniswe okufunayo.amaprothenikokunye ukungcola.Ngokuvamile, lesi sinyathelo sokuhlukanisa sisebenzisa izindlela ezifana nokukhipha usawoti, ukuqoqwa kwephuzu le-isoelectric kanye nokuhlukaniswa kwe-organic solvent.Lezi zindlela zibonakala ngokulula kanye nomthamo omkhulu wokucubungula, ongasusa ukungcola okuningi futhi ugxilise isisombululo samaprotheni.Okunye okukhishwe kwamaprotheni kukhulu ngevolumu futhi akufanelekile ukugxiliswa ngokunqwabelana noma ukukhipha usawoti.Ungakhetha i-ultrafiltration, i-gel filtration, okomisa i-vacuum eqandisiwe noma ezinye izindlela zokugxilisa ingqondo.

Ukuhlukana okuhle

Ngemuva kokuhlukaniswa okuqinile kwesampula, ivolumu ngokuvamile iba mncane, futhi ukungcola okuningi kususiwe.Ukuze uthole ukuhlanzwa okwengeziwe, izindlela zechromatography ngokuvamile zihlanganisa ukuhlungwa kwejeli, i-ion exchange chromatography, i-adsorption chromatography, kanye ne-affinity chromatography.Uma kunesidingo, ungaphinda ukhethe i-electrophoresis, kuhlanganise ne-zone electrophoresis, isethi ye-isoelectric point, njll njengenqubo yokugcina yokuhlanza.Indlela esetshenziswa ekuhlukaniseni izinga lokuhlukanisa ngokuvamile incane ekuhleleni, kodwa inokulungiswa okuphezulu.

I-Crystallization iyinqubo yokugcina yokuhlukaniswa kwamaprotheni kanye nokuhlanzwa.Nakuba inqubo ye-crystallization ingaqinisekisi ukuthi amaprotheni kufanele afane, kuphela lapho iphrotheni ethile inenzuzo kwisixazululo sokwenza i-crystal.Inqubo ye-crystallization ngokwayo ihambisana nezinga elithile lokuhlanzwa, futhi ukuvuselela kabusha kungasusa inani elincane lamaprotheni aphikisiwe.Kusukela denaturedamaprotheniengakaze itholakale ngesikhathi senqubo ye-crystallization, i-protein crystallization ayiyona nje isibonakaliso sokuhlanzeka, kodwa futhi isiqondiso esinamandla sokunquma ukuthi umkhiqizo usesimweni sawo semvelo.