Ukwahlula kunye nokuhlanjululwa kweeprotheni zisetyenziswa ngokubanzi kuphando lwe-biochemistry kunye nokusetyenziswa kwaye sisakhono esibalulekileyo sokusebenza.I-SCG yeProtein Purification System Company-Saipu Instrument iye yaqulunqa ukuhlukana okungcolileyo kunye nomxholo wokwahlula okulungileyo.iprotheniukuhlanjululwa komntu wonke.Iseli eqhelekileyo ye-eukaryotic inokuqulatha amawaka eeprotheyini ezahlukeneyo, ezinye zizityebi kakhulu kwaye ezinye zineekopi ezimbalwa kuphela.Ukuze ufunde iprotheni ethile, kuyimfuneko ukuqala ukuhlambulula iprotheni evela kwezinye iiprotheni kunye neemolekyuli ezingenazo iiprotheni.

Ukwahlula okurhabaxa

Xa i-protein extract (ngamanye amaxesha ixutywe kunye ne-nucleic acids, i-polysaccharides, njl.)iprothenikobunye ukungcola.Ngokubanzi, eli nyathelo lokwahlula lisebenzisa iindlela ezinje ngetyuwa ngaphandle, ukuqokelelwa kwamanqaku e-isoelectric kunye nokuqhekeka kwe-solvent ye-organic.Ezi ndlela zibonakaliswe ngokulula kunye nomthamo omkhulu wokucubungula, onokususa ukungcola okuninzi kwaye ugxininise isisombululo seprotheni.Ezinye izicatshulwa zeprotheyini zinkulu ngomthamo kwaye azifanelekanga ukugxininiswa ngokuqokelela okanye ukutyumba ngaphandle.Unokukhetha i-ultrafiltration, i-gel filtration, i-vacuum epholileyo yokumisa okanye ezinye iindlela zokuxilisa.

Ukwahlula okulungileyo

Emva kokuqhekeka okukrakra kwesampulu, umthamo uncinci, kwaye ubuninzi bokungcola bususiwe.Ukucocwa ngakumbi, iindlela zechromatography ngokubanzi zibandakanya ukuhluzwa kwejeli, ukutshintshiselana nge-ion chromatography, i-adsorption chromatography, kunye ne-affinity chromatography.Ukuba kuyimfuneko, unokukhetha kwakhona i-electrophoresis, kubandakanywa i-electrophoresis yendawo, i-isoelectric point set, njl. njengenkqubo yokugqibela yokucoca.Indlela esetyenziswayo yokwahlula inqanaba lolwahlulo idla ngokuba yincinci kucwangciso, kodwa inesisombululo esiphezulu.

I-Crystallization yinkqubo yokugqibela yokuhlukana kweeprotheni kunye nokuhlanjululwa.Nangona inkqubo ye-crystallization ayiqinisekisi ukuba iprotheni kufuneka ibe yinto efanayo, kuphela xa iprotheni ethile inenzuzo kwisisombululo sokwenza i-crystal.Inkqubo ye-crystallization ngokwayo ihamba kunye neqondo elithile lokuhlanjululwa, kwaye i-recrystallization inokususa inani elincinci leprotheni egqwethiweyo.Ekubeni denaturediprotheniayizange ifunyanwe ngexesha lenkqubo ye-crystallization, i-protein crystallization ayilona nje uphawu lokucoceka, kodwa kunye nesikhokelo esinamandla sokugqiba ukuba imveliso ikwimeko yendalo.