Uburyo bwakweza poroteyine:

Uburyo bwo kweza poroteyine, gutandukanya no kweza poroteyine, poroteyine irekurwa mu ngirabuzimafatizo cyangwa ingirangingo z'umwimerere mu buryo bwashonze kandi ikaguma mu mwimerere karemano nta gutakaza ibikorwa by’ibinyabuzima.Kubera iyo mpamvu, ibikoresho bigomba kuvanaho inyamanswa na adipose, ibikoresho byimbuto bigomba no gukuraho kwanduza ikoti ryimbuto mubintu nka tannine, kandi imbuto yamavuta igomba guhitamo gukoresha ibishishwa bito bitetse.

1. Uburyo bwo kweza poroteyine, ingano nuburyo butandukanye bwo gusesengura gutandukana: dialyse na ultrafiltration bakoresha izo poroteyine kugirango zinyure muri kamere ya molekile idashobora kunyura muri kimwe cya kabiri cyinjira;density gradient centrifugation Ubwiza nubucucike bwa poroteyine hagati bizagira ingaruka zikomeye kubice.Gukemura umuvuduko birihuta;gel muyunguruzi ni ubwoko bwinkingi chromatografiya.

2. Gutandukanya itandukaniro rya solubilité mugukoresha: imvura-mvura kuko net net ya molekile ya proteine ​​iri kuri zero isoelectric point, bikagabanya kwanga electrostatike hagati ya molekile, byoroshye kwegeranya, hamwe nubushake buke;igisubizo cyumunyu gikoreshwa numuti wumunyu Ubwiyongere bwiyongera cyangwa bugabanya gukomera kwa poroteyine.

3. Amafaranga yishyurwa aratandukanye kandi asesengurwa nuburyo butandukanye, ibyingenzi birimo electrophoreis na ion yo guhana chromatografiya.

4. Uburyo bwakweza poroteyineGuhitamo adsorption no gutandukanya poroteyine zikoresha imbaraga za adsorption.

5. Affinity chromatography ishingiye ku gutandukanya ligande.Iyi miterere iranga ibinyabuzima ituma molekile za poroteyine zihuza cyane aho guhurirana nindi molekile yitwa ligand.

6Ubu buryo buri hejuru kurenza imyunyu ngugu, ariko poroteyine yerekana byinshi Iyo byoroshye, bigomba gukorwa mubushyuhe buke.

Uburyo bwo kwezakweza poroteyine:

Nigute ushobora kweza poroteyine, proteyine ni molekile nini, hamwe nubunini butandukanye bwa alubumu, hariho uburyo bworoshye bwo gutandukanya poroteyine nibikoresho bito bya molekile, ndetse no gutandukanya poroteyine.Uburyo nyamukuru bwo gutandukanya poroteyine zingana na molekuline zitandukanye ni dialyse, ultrafiltration, gel filtration, hamwe no gutandukanya centrifugal.Dialysis na ultrafiltration bikoreshwa muburyo bwo gutandukanya poroteyine.Uruvangitirane rwa dialyse rugomba gutandukana rushyirwa mumufuka wa dialyse wakozwe na semipermeable membrane, hanyuma ukinjizwa mumuti wa dialyse kugirango utandukane.Ultrafiltration ninzira yo gutambutsa amazi nizindi molekile ntoya ukoresheje imbaraga za centrifugal cyangwa igitutu muri kimwe cya kabiri cyinjira hamwe no gufata proteine ​​mugice cya kabiri cyinjira.Ubu buryo bubiri bushobora gutandukanywa na macromolecules ishingiye kuri poroteyine na molekile ntoya ishingiye ku munyu.Bakunze kuba umunyu, ugakoreshwa muburyo bwo gusesa umunyu, cyangwa urashobora gutangizwa kugirango ukoreshe ubu buryo bubiri nyuma yo gushiramo umunyu ukoresheje ibisubizo byumunyu ngugu.

1. Nigute ushobora kweza poroteyine ukoresheje uburyo bwo gusohora: Intandaro yuburyo bwo gusohora ni uko gukomera kwa poroteyine mugisubizo cyumunyu mwinshi biziyongera hamwe no kwiyongera kwumunyu mwinshi, ariko mugihe umunyu mwinshi wiyongereye kugeza ku gaciro runaka, ibikorwa by'amazi biziyongera.Kugabanuka, ibyo na byo bikaba bishobora gutuma poroteyine zimwe na zimwe zitanga amafaranga binyuze mu miterere ya molekuline igenda itabangikanya buhoro buhoro, kandi na hydration membrane igenda isenywa buhoro buhoro, amaherezo bikaba bishobora gutera poroteyine na molekile bifitanye isano no gukura hamwe no guturuka ku bisubizo bitandukanye.

2. Uburyo bwimvura igwa: Hariho impamvu ebyiri zo kugabanya ubukana bwa poroteyine mumashanyarazi kama: uburyo bwo kweza poroteyine nkigisubizo cyumunyu hamwe no kubura umwuma;icya kabiri, dielectric ihoraho yumuti ukomoka kumazi kuruta amazi irashobora kugabanuka Mumashanyarazi.

3. Intungamubiri za poroteyine: poroteyine nk'imvura igwa ikora gusa ubwoko bumwe cyangwa bumwe mu mvura ya poroteyine, ibisanzwe ni alkaline, proteyine, insiguro, hamwe n'ibyuma biremereye.

4. Imvura ya polyethylene glycol: Nigute ushobora kweza amazi adashonga amazi atari ionic polymers kurikweza poroteyine, nka polyethylene glycol na sodium dextran sulfate kugirango igabanye poroteyine.